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RALBP1 Mouse mAb

COA COA
    应用:
  • IF/ICC
  • IHC
  • IP
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Mouse IgG1
  • 克隆号: OTI2A6
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab124484-10μl
 10μl 现货 Stock Image
Ab124484-50μl
 50μL 现货 Stock Image
Ab124484-100μl
 100μL 期货 Stock Image
Ab124484-1ml
 1ml 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
查看相关系列
点击查看靶蛋白信息: RALBP1
Accession#:Q15311 (1) Gene ID:10928 (1) RALBP1 (1)
main product photo

基本信息

产品名称 RALBP1 Mouse mAb
别名 RALBP1抗体 | RalA结合蛋白1抗体 | RALBP1抗体 | RalA结合蛋白1抗体 | RALBP1抗体 | RALBP1单克隆抗体 | RalA结合蛋白1抗体
英文别名 RLIP1 antibody | 76 kDa Ral-interacting protein antibody | 76-kDa Ral-interacting protein antibody | Dinitrophenyl S-glutathione ATPase antibody | DNP-SG ATPase antibody | Ral-interacting protein 1 antibody | Ral-interacting protein 1, 76-KD antibody | Ra
规格或纯度 敲除验证, ExactAb™, 已验证, 见COA
生化机理 Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
宿主种属 小鼠(Mouse)
特异性 RALBP1
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 Recombinant f ull length protein of human RALBP1.
阳性对照 WB: HeLa, Hep G2, MCF7, HT-29, NIH/3T3 and C6 cell lysates. IHC: Human breast adenocarcinoma, colon adenocarcinoma, kidney, ovary adenocarcinoma, endometrium adenocarcinoma, prostate, prostate carcinoma tissues. IF/ICC: COS7 cells transiently transfected
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 1% BSA, 0.02% Sodium azide, pH 7.3
防腐剂 0.02% Sodium azide
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点（人）

RALBP1 Tbio RalA 结合蛋白 1(RalA-binding protein 1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

RALBP1 Mouse mAb (Ab124484) - Western Blot
All lanes: RALBP1 Mouse mAb (Ab124484) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 76 kDa
Observed band size: 91 kDa
Exposure time: 60.0 s

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of breast tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of colon tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Kidney tissue within the normal limits using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of ovary tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50)

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of endometrium tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded prostate tissue within the normal limits using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of prostate tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

RALBP1 Mouse mAb (Ab124484) - IF/ICC
RALBP1 Mouse mAb (Ab124484) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1.

RALBP1 Mouse mAb (Ab124484) - IF/ICC
Immunofluorescent staining of Hep G2 cells using RALBP1 Mouse mAb (Ab124484).

RALBP1 Mouse mAb (Ab124484) - KO
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and RALBP1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with RALBP1 Mouse mAb (Ab124484) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

应用

应用名称 稀释比例
WB

1/1000

IF/ICC

1/100
IHC

1/50
IP

4 μg/mL

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找到2个结果

批号(Lot Number) 证书类型 货号
ZJ25F0117317 分析证书 Ab124484
ZJ25F0117318 分析证书 Ab124484

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引用文献

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
2. Jullien-Flores, V V and 8 more authors..  (1995)  Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity..  The Journal of biological chemistry,  (22): [PMID:7673236]
3. Ikeda, M M, Ishida, O O, Hinoi, T T, Kishida, S S and Kikuchi, A A..  (1998)  Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of Ral..  The Journal of biological chemistry,  (9): [PMID:9422736]
4. Jullien-Flores, V V and 6 more authors..  (2000)  RLIP76, an effector of the GTPase Ral, interacts with the AP2 complex: involvement of the Ral pathway in receptor endocytosis..  Journal of cell science,  [PMID:10910768]
5. Awasthi, S S and 9 more authors..  (2000)  Novel function of human RLIP76: ATP-dependent transport of glutathione conjugates and doxorubicin..  Biochemistry,  (8): [PMID:10924126]
6. Sharma, R R and 7 more authors..  (2001)  RLIP76 is the major ATP-dependent transporter of glutathione-conjugates and doxorubicin in human erythrocytes..  Archives of biochemistry and biophysics,  (15): [PMID:11437348]
7. Rossé, Carine C, L'Hoste, Sébastien S, Offner, Nicolas N, Picard, André A and Camonis, Jacques J..  (2003)  RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis..  The Journal of biological chemistry,  (15): [PMID:12775724]
8. Yadav, Sushma S and 7 more authors..  (2004)  Identification of membrane-anchoring domains of RLIP76 using deletion mutant analyses..  Biochemistry,  (28): [PMID:15610018]
9. Zahedi, René P RP and 8 more authors..  (2008)  Phosphoproteome of resting human platelets..  Journal of proteome research,  [PMID:18088087]
10. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,  (18): [PMID:19690332]
11. Fenwick, R Brynmor and 7 more authors..  (2010)  The RalB-RLIP76 complex reveals a novel mode of ral-effector interaction..  Structure (London, England : 1993),  (11): [PMID:20696399]
12. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,  (15): [PMID:21406692]
13. Kashatus, David F DF and 5 more authors..  (2011)  RALA and RALBP1 regulate mitochondrial fission at mitosis..  Nature cell biology,  (7): [PMID:21822277]
14. Fillatre, Jonathan J and 7 more authors..  (2012)  Dynamics of the subcellular localization of RalBP1/RLIP through the cell cycle: the role of targeting signals and of protein-protein interactions..  FASEB journal : official publication of the Federation of American Societies for Experimental Biology,  [PMID:22319010]
15. Rajasekar, Karthik V; Campbell, Louise J; Nietlispach, Daniel; Owen, Darerca and Mott, Helen R..  (2013)  The structure of the RLIP76 RhoGAP-Ral binding domain dyad: fixed position of the domains leads to dual engagement of small G proteins at the membrane..  Structure (London, England : 1993),  (3): [PMID:24207123]

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