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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab121265-10μl |
10μl |
现货 ![]() |
| |
| Ab121265-50μl |
50μL |
现货 ![]() |
| |
| Ab121265-100μl |
100μL |
期货 ![]() |
| |
| Ab121265-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Perilipin 3/TIP47 Mouse mAb |
|---|---|
| 别名 | PLIN3抗体 | PLIN3单抗 | PLIN3鼠单抗 |
| 英文别名 | Cargo selection protein TIP47 | M6PRBP1 | M6PRBP1MGC2012 | Mannose-6-phosphate receptor-binding protein 1 | perilipin 3,47 kDa MPR-binding protein | Perilipin3 | Perilipin-3 | Placental protein 17,47 kDa mannose 6-phosphate receptor-binding protein | PLIN |
| 规格或纯度 | ExactAb™, 已验证, 无载体, 0.5 mg/mL |
| 生化机理 | 需要将 6-磷酸甘露糖受体(MPR)从内体运输到跨高尔基网络。 |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | PLIN3 |
| 种属反应性 | 人(Human) |
| 免疫原 | KLH-conjugated synthetic peptide of human TIP47. |
| 阳性对照 | WB: K562 and U-2 OS cell lysates. IF/ICC: U-2 OS cells |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Mouse IgG2a |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein G purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 30% Glycerol, 0.01% Sodium azide, pH 7.3 |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.5 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4℃ 短期储存(1-2 周)。-20℃长期保存(12 个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
Perilipin 3/TIP47 Mouse mAb (Ab121265) - Western Blot
All lanes: Perilipin 3/TIP47 Mouse mAb (Ab121265) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 126.3 s
Perilipin 3/TIP47 Mouse mAb (Ab121265) - IF/ICC
Immunofluorescent analysis of TIP47 staining in U-2 OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Perilipin 3/TIP47 Mouse mAb (Ab121265) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC |
1/10-1/50
|
| WB |
1/500-1/1000
|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab121265 | |
| 分析证书 | Ab121265 |