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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab120677-10μl |
10μl |
现货 ![]() |
| |
| Ab120677-50μl |
50μL |
现货 ![]() |
| |
| Ab120677-100μl |
100μL |
期货 ![]() |
| |
| Ab120677-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | PCMT1 Mouse mAb |
|---|---|
| 别名 | PCMT1抗体 | PCMT1单克隆抗体 | 蛋白L-异天冬氨酸-O-甲基转移酶抗体 |
| 英文别名 | L isoaspartyl protein carboxyl methyltransferase antibody | L isoaspartyl/D aspartyl methyltransferase antibody | L-isoaspartyl protein carboxyl methyltransferase antibody | PCMT 1 antibody | Pcmt1 antibody | PIMT antibody | PIMT_HUMAN antibody | Protein |
| 规格或纯度 | ExactAb™, 已验证, 见COA |
| 生化机理 | Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | PCMT1 |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 免疫原 | Recombinant protein of human PCMT1 (AA 60-285). |
| 阳性对照 | WB: HeLa, Jurkat, MCF7, K562, NIH/3T3 and C6 cell lysates. IHC: Human breast, colon, kidney, lung, pancreas tissues. |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 1% BSA, 0.02% sodium azide. pH 7.3. |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 运输。收货后建议分装。储存于 -20°C 或 -80°C。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
PCMT1 Mouse mAb (Ab120677) - Western Blot
All lanes: PCMT1 Mouse mAb (Ab120677) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 3.5 s
PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human breast tissue tissue using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).
PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human colon tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).
PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human Kidney tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, Ab120677) (1/2000).
Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human lung tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).
PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).
| 应用名称 | 稀释比例 |
|---|---|
| WB | 1/500-1/2000 |
| IHC | 1/2000 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab120677 | |
| 分析证书 | Ab120677 |