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PCMT1 Mouse mAb

    应用:
  • IHC
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Mouse IgG2b
  • 克隆号: OTI2A9
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab120677-10μl
10μl 现货 Stock Image
Ab120677-50μl
50μL 现货 Stock Image
Ab120677-100μl
100μL 期货 Stock Image
Ab120677-1ml
1ml 期货 Stock Image

基本信息

产品名称 PCMT1 Mouse mAb
别名 PCMT1抗体 | PCMT1单克隆抗体 | 蛋白L-异天冬氨酸-O-甲基转移酶抗体
英文别名 L isoaspartyl protein carboxyl methyltransferase antibody | L isoaspartyl/D aspartyl methyltransferase antibody | L-isoaspartyl protein carboxyl methyltransferase antibody | PCMT 1 antibody | Pcmt1 antibody | PIMT antibody | PIMT_HUMAN antibody | Protein
规格或纯度 ExactAb™, 已验证, 见COA
生化机理 Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.
宿主种属 小鼠(Mouse)
特异性 PCMT1
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 Recombinant protein of human PCMT1 (AA 60-285).
阳性对照 WB: HeLa, Jurkat, MCF7, K562, NIH/3T3 and C6 cell lysates. IHC: Human breast, colon, kidney, lung, pancreas tissues.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
Format Whole IgG
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 1% BSA, 0.02% sodium azide. pH 7.3.
防腐剂 0.02% Sodium azide
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 运输。收货后建议分装。储存于 -20°C 或 -80°C。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

PCMT1 Tbio 蛋白质-L-异天冬氨酸(D-天冬氨酸)O-甲基转移酶(Protein-L-isoaspartate(D-aspartate) O-methyltransferase) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

PCMT1 Mouse mAb (Ab120677) - Western Blot
All lanes: PCMT1 Mouse mAb (Ab120677) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 3.5 s

PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human breast tissue tissue using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).

PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human colon tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).

PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human Kidney tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, Ab120677) (1/2000).

Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human lung tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).

PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).

应用

应用名称 稀释比例
WB

1/500-1/2000

IHC

1/2000

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批号(Lot Number) 证书类型 货号
ZJ25F0724824 分析证书 Ab120677
ZJ25F0724825 分析证书 Ab120677

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