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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab112720-10μl |
10μl |
现货 ![]() |
| |
| Ab112720-50μl |
50μL |
现货 ![]() |
| |
| Ab112720-100μl |
100μL |
期货 ![]() |
| |
| Ab112720-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Recombinant Laminin gamma 1 Antibody |
|---|---|
| 别名 | 层粘连蛋白gamma1重组兔单克隆抗体 |
| 英文别名 | LAMB2 antibody | lamc1 antibody | LAMC1_HUMAN antibody | Laminin B2 chain antibody | Laminin subunit gamma-1 antibody | Laminin, gamma 1 (formerly LAMB2) antibody | Laminin-1 subunit gamma antibody | Laminin-10 subunit gamma antibody | Laminin-11 subunit |
| 规格或纯度 | 重组, ExactAb™, 敲低验证, 已验证, 见COA |
| 生化机理 | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | Laminin gamma 1 |
| 种属反应性 | 人(Human) |
| 免疫原 | A synthetic peptide derived from human Laminin gamma 1 (AA 10-75) |
| 阳性对照 | WB: A431 and HEK293 cell lysates. |
| 偶联 | Unconjugated |
| 克隆类型 | 重组抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4 |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Recombinant Laminin gamma 1 Antibody (Ab112720) - Western Blot
All lanes: Recombinant Laminin gamma 1 Antibody (Ab112720) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 178 kDa
Observed band size: 250 kDa
Exposure time: 200.0 s
Recombinant Laminin gamma 1 Antibody (Ab112720) - Western Blot
All lanes: Recombinant Laminin gamma 1 Antibody (Ab112720) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 178 kDa
Observed band size: 250 kDa
Exposure time: 94.4 s
Recombinant Laminin gamma 1 Antibody (Ab112720) - Western Blot
All lanes use Recombinant Laminin gamma 1 Antibody (Ab112720) at 1/1000 dilution for 1 hour at room temperature.
| 应用名称 | 稀释比例 |
|---|---|
| WB | 1/1000 - 1/2000 |
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab112720 | |
| 分析证书 | Ab112720 |
| 1. Santos, C L CL, Sabbaga, J J and Brentani, R R.. (1991) Differences in human laminin B2 sequences.. DNA sequence : the journal of DNA sequencing and mapping, [PMID:1806043] |
| 2. Kallunki, T T, Ikonen, J J, Chow, L T LT, Kallunki, P P and Tryggvason, K K.. (1991) Structure of the human laminin B2 chain gene reveals extensive divergence from the laminin B1 chain gene.. The Journal of biological chemistry, (5): [PMID:1985895] |
| 3. Fukushima, Y Y and 8 more authors.. (1988) Isolation of a human laminin B2 (LAMB2) cDNA clone and assignment of the gene to chromosome region 1q25----q31.. Cytogenetics and cell genetics, [PMID:3234037] |
| 4. Pikkarainen, T T, Kallunki, T T and Tryggvason, K K.. (1988) Human laminin B2 chain. Comparison of the complete amino acid sequence with the B1 chain reveals variability in sequence homology between different structural domains.. The Journal of biological chemistry, (15): [PMID:3360804] |
| 5. Zhang, Hui H, Li, Xiao-Jun XJ, Martin, Daniel B DB and Aebersold, Ruedi R.. (2003) Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.. Nature biotechnology, [PMID:12754519] |
| 6. Liu, Tao T and 6 more authors.. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952] |
| 7. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414] |
| 8. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974] |
| 9. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 10. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912] |
| 11. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039] |
| 12. Harripaul, R R and 31 more authors.. (2018) Mapping autosomal recessive intellectual disability: combined microarray and exome sequencing identifies 26 novel candidate genes in 192 consanguineous families.. Molecular psychiatry, [PMID:28397838] |
| 13. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |