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GCSF Receptor Antibody

GCSF Receptor Antibody

级别和纯度:

见COA

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab105080-10μl	10μl	现货
Ab105080-50μl	50μL	现货
Ab105080-100μl	100μL	现货
Ab105080-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: CSF3R

Accession#:Q99062 (2) CD114 (2) CSF3R (2) G-CSFR (2) Gene ID:1441 (2)

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基本信息

产品名称	GCSF Receptor Antibody
别名	粒细胞-巨噬细胞集落刺激因子3受体抗体 \| GCSF 受体抗体
英文别名	CD114 \| G-CSFR \| CSF3R \| CSF3-R \| GCSF-R \| CD 114 antibody \| CD114 antibody \| CD114 antigen antibody \| Colony stimulating factor 3 receptor (granulocyte) antibody \| Colony stimulating factor 3 receptor antibody \| CSF 3R antibody \| CSF3R antibody \| CSF3R_H
规格或纯度	无载体, ExactAb™, 已验证, 高性能, 见COA
宿主种属	兔(Rabbit)
特异性	CSF3R
种属反应性	人(Human)
免疫原	Recombinant GCSFR expressed in E.coli (AA 26-138).
阳性对照	WB: THP1, MCF7, K562, Hep G2 and U937 cell lysates. IHC: Human placenta, appendix, stomach cancer, colorectal cancer tissues. ICC/IF: MCF7 cells.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified + Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH 7.4
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

CSF3R Tclin 粒细胞集落刺激因子受体(Granulocyte colony-stimulating factor receptor) (0 活性数据)

点击查看靶蛋白信息： CSF3R

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: GCSF Receptor Antibody (Ab105080)

GCSF Receptor Antibody (Ab105080) - Western Blot
All lanes: GCSF Receptor Antibody (Ab105080) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 85 kDa
Observed band size: 78 kDa
Exposure time: 3.4 s

IF/ICC: GCSF Receptor Antibody (Ab105080)

GCSF Receptor Antibody (Ab105080) - IF/ICC
FITC staining on IF
Sample: MCF7 cells
Primary Ab: 20 µg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GCSF Receptor Antibody (Ab105080)

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GCSF Receptor Antibody (Ab105080)

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Appendix Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GCSF Receptor Antibody (Ab105080)

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Stomach cancer Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GCSF Receptor Antibody (Ab105080)

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Colorectal cancer Tissue
Primary Ab: 10 μg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GCSF Receptor Antibody (Ab105080)

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: GCSF Receptor Antibody (Ab105080)

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-20 µg/mL
IF/ICC	5-20 µg/mL
WB	0.01-2 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1101531	分析证书	Ab105080
ZJ23F1101532	分析证书	Ab105080
ZJ24F1011835	分析证书	Ab105080

技术文档和文章

石蜡样品的免疫组化染色方案（IHC-P）

Categories: 实验方案（Protocols）

探索主题:

单克隆抗体石蜡切片免疫组化染色

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

免疫荧光染色实验步骤（甲醇通透法）

Categories: 实验方案（Protocols）

探索主题:

冷冻切片免疫荧光染色组织切片甲醇通透 IF

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

引用文献

1. Tamada T, Honjo E, Maeda Y, Okamoto T, Ishibashi M, Tokunaga M, Kuroki R. (2006) Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex.. Proc Natl Acad Sci USA, 103 (9): (3135-40). [PMID:16492764]
2. Seto, Y Y, Fukunaga, R R and Nagata, S S.. (1992) Chromosomal gene organization of the human granulocyte colony-stimulating factor receptor.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:1530796]
3. Fukunaga, R R, Seto, Y Y, Mizushima, S S and Nagata, S S.. (1990) Three different mRNAs encoding human granulocyte colony-stimulating factor receptor.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:1701053]
4. Fukunaga, R R, Ishizaka-Ikeda, E E, Pan, C X CX, Seto, Y Y and Nagata, S S.. (1991) Functional domains of the granulocyte colony-stimulating factor receptor.. The EMBO journal, [PMID:1717255]
5. Larsen, A A and 9 more authors.. (1990) Expression cloning of a human granulocyte colony-stimulating factor receptor: a structural mosaic of hematopoietin receptor, immunoglobulin, and fibronectin domains.. The Journal of experimental medicine, (1): [PMID:2147944]
6. Dong, F F and 7 more authors.. (1994) Identification of a nonsense mutation in the granulocyte-colony-stimulating factor receptor in severe congenital neutropenia.. Proceedings of the National Academy of Sciences of the United States of America, (10): [PMID:7514305]
7. Haniu, M M and 5 more authors.. (1995) Extracellular domain of granulocyte-colony stimulating factor receptor. Interaction with its ligand and identification of a domain in close proximity of ligand-binding region.. Archives of biochemistry and biophysics, (20): [PMID:8554326]
8. Yamasaki, K K, Naito, S S, Anaguchi, H H, Ohkubo, T T and Ota, Y Y.. (1997) Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand.. Nature structural biology, [PMID:9187659]
9. Layton, J E JE, Iaria, J J, Smith, D K DK and Treutlein, H R HR.. (1997) Identification of a ligand-binding site on the granulocyte colony-stimulating factor receptor by molecular modeling and mutagenesis.. The Journal of biological chemistry, (21): [PMID:9368043]
10. Ward, A C AC and 7 more authors.. (1999) Novel point mutation in the extracellular domain of the granulocyte colony-stimulating factor (G-CSF) receptor in a case of severe congenital neutropenia hyporesponsive to G-CSF treatment.. The Journal of experimental medicine, (16): [PMID:10449521]
11. Elsässer, Annika A and 12 more authors.. (2003) The fusion protein AML1-ETO in acute myeloid leukemia with translocation t(8;21) induces c-jun protein expression via the proximal AP-1 site of the c-jun promoter in an indirect, JNK-dependent manner.. Oncogene, (28): [PMID:12944913]
12. Cao, Yan-Ran YR and 11 more authors.. (2005) [The response of bone marrow hematopoietic cells to G-CSF in paroxysmal nocturnal hemoglobinuria patients].. Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi, [PMID:15949269]
13. Zhuang, Dazhong D, Qiu, Yaling Y, Haque, S Jaharul SJ and Dong, Fan F.. (2005) Tyrosine 729 of the G-CSF receptor controls the duration of receptor signaling: involvement of SOCS3 and SOCS1.. Journal of leukocyte biology, [PMID:16033816]
14. Erkeland, S J SJ and 9 more authors.. (2007) Novel role of WD40 and SOCS box protein-2 in steady-state distribution of granulocyte colony-stimulating factor receptor and G-CSF-controlled proliferation and differentiation signaling.. Oncogene, (29): [PMID:17001306]
15. and Ward, Alister C AC.. (2007) The role of the granulocyte colony-stimulating factor receptor (G-CSF-R) in disease.. Frontiers in bioscience : a journal and virtual library, (1): [PMID:17127322]
16. Link, Daniel C DC and 28 more authors.. (2007) Distinct patterns of mutations occurring in de novo AML versus AML arising in the setting of severe congenital neutropenia.. Blood, (1): [PMID:17494858]
17. Zeidler, Cornelia C, Germeshausen, Manuela M, Klein, Christoph C and Welte, Karl K.. (2009) Clinical implications of ELA2-, HAX1-, and G-CSF-receptor (CSF3R) mutations in severe congenital neutropenia.. British journal of haematology, [PMID:19120359]
18. Plo, Isabelle I and 11 more authors.. (2009) An activating mutation in the CSF3R gene induces a hereditary chronic neutrophilia.. The Journal of experimental medicine, (3): [PMID:19620628]
19. Triot, Alexa A and 20 more authors.. (2014) Inherited biallelic CSF3R mutations in severe congenital neutropenia.. Blood, (12): [PMID:24753537]
20. Klimiankou, Maksim M and 8 more authors.. (2015) GM-CSF stimulates granulopoiesis in a congenital neutropenia patient with loss-of-function biallelic heterozygous CSF3R mutations.. Blood, (8): [PMID:26324699]

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