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Recombinant CYP2D6 Antibody

    应用:
  • IF/ICC
  • IHC
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab098589-10μl
10μl 现货 Stock Image
Ab098589-50μl
50μL 现货 Stock Image
Ab098589-100μl
100μL 期货 Stock Image
Ab098589-1ml
1ml 期货 Stock Image

基本信息

产品名称 Recombinant CYP2D6 Antibody
别名 细胞色素P450 2D6重组兔单克隆抗体 | 细胞色素P450 2D6重组兔单克隆抗体
英文别名 Cholesterol 25-hydroxylase antibody | CP2D6_HUMAN antibody | CPD6 antibody | CYP2D antibody | CYP2D6 antibody | CYP2D7AP antibody | CYP2D7BP antibody | CYP2D7P2 antibody | CYP2D8P2 antibody | CYP2DL1 antibody | CYPIID6 antibody | Cytochrome P450 2D6 antib
规格或纯度 重组, ExactAb™, 已验证, 0.5 mg/mL
生化机理 Responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It is involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants.
宿主种属 兔(Rabbit)
特异性 CYP2D6
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 Recombinant protein of human CYP2D6.
阳性对照 WB: Mouse liver, Rat liver lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells.
偶联 Unconjugated

产品属性

克隆类型 重组抗体
Format Whole IgG
亚型 Rabbit IgG
纯化方法 Immunogen affinity purified
物理外观 Liquid
储存缓冲液 50mM Tris-Glycine, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂 0.01% Sodium azide
浓度 0.5 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

CYP2D6 Tclin 细胞色素 P450 2D6(Cytochrome P450 2D6) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

Recombinant CYP2D6 Antibody (Ab098589) - Western Blot
All lanes: Recombinant CYP2D6 Antibody (Ab098589) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 56 kDa
Observed band size: 48 kDa
Exposure time: 3.1 s

Recombinant CYP2D6 Antibody (Ab098589) - IHC
Immunohistochemical analysis of Cytochrome P450 2D6 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant CYP2D6 Antibody (Ab098589) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Recombinant CYP2D6 Antibody (Ab098589) - IF/ICC
Immunofluorescent analysis of Cytochrome P450 2D6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Recombinant CYP2D6 Antibody (Ab098589) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

应用

应用名称 稀释比例
WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/50-1/100

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批号(Lot Number) 证书类型 货号
ZJ25F0117360 分析证书 Ab098589
ZJ25F0117359 分析证书 Ab098589

引用文献

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