基本信息

产品名称	CD86 Rat mAb
别名	CD86单克隆抗体 \| Anti-CD86抗体 \| CD86抗体 \| CD86单抗 \| CD86大鼠单克隆抗体 \| CD86大鼠单抗
英文别名	Activation B7-2 antigen \| B70 \| B7-2 antigen \| B72 \| B7-2 \| B-lymphocyte activation antigen B7-2 \| BU63 \| CD28 antigen ligand 2 \| CD28LG2B7-2 antigen) \| CD86 antigen \| CD86 molecule \| CD86 \| CTLA-4 counter-receptor B7.2 \| FUN-1 \| LAB72 \| MGC34413 \| T-lymp
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
生化机理	Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
宿主种属	大鼠(Rat)
特异性	CD86
种属反应性	小鼠(Mouse)
免疫原	LPS-activated CBA/Ca mouse splenic B cells
阳性对照	Flow: Mouse splenocytes.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	GL1
Format	Whole IgG
亚型	Rat IgG2a
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, pH 7.2-7.4
防腐剂	No
浓度	见COA
储存温度	2-8°C储存,禁止冷冻
运输条件	冰袋运输
稳定性与储存	Store at 2-8°C. Store at -20°C for long term. Upon delivery aliquot. Avoid freeze/thaw cycle.
分子类型	抗体

关联靶点（人）

CD86 Tclin T淋巴细胞活化抗原CD86(T-lymphocyte activation antigen CD86) (0 活性数据)

点击查看靶蛋白信息： CD86

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: CD86 Rat mAb (Ab095462)

CD86 Rat mAb (Ab095462) - Flow Cytometry
Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 (red) with CD86 Rat mAb (Ab095462) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD86 Rat mAb (Ab095462)

CD86 Rat mAb (Ab095462) - Flow Cytometry
Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 with CD86 Rat mAb (Ab095462) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156150).

应用

应用名称	稀释比例
Flow Cytometry	0.25μg/100w cells
WB	Use at an assay dependent concentration.
Blocking	Use at an assay dependent concentration.
Functional Assay	Use at an assay dependent concentration.
IP	Use at an assay dependent concentration.
ELISA	Use at an assay dependent concentration.
Animal Model	Use at an assay dependent concentration.
IHC-F	Use at an assay dependent concentration.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0505479	分析证书	Ab095462
ZJ24F0505480	分析证书	Ab095462
ZJ24F0505481	分析证书	Ab095462

技术文档和文章

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection