BECN1 Mouse mAb

COA

级别和纯度:

无载体

已验证

ExactAb™

1.0mg/mL

应用:

ELISA
Flow Cytometry
IHC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: 2A4
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab090922-10μl	10μl	现货
Ab090922-50μl	50μL	现货
Ab090922-100μl	100μL	现货
Ab090922-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: BECN1

Accession#:Q14457 (4) Beclin 1 (2) BECN1 (4) Gene ID:8678 (3) 流式细胞术 (1163)

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基本信息

产品名称	BECN1 Mouse mAb
别名	BECN1 小鼠单克隆抗体 \| BECN1 小鼠单抗 \| BECN1抗体 \| BECN1单克隆抗体 \| Beclin 1抗体 \| 苄氯素1抗体 \| Beclin 1单克隆抗体
英文别名	Coiled-coil myosin-like BCL2-interacting protein \| Protein GT197 \| APG6 antibody \| ATG 6 antibody \| ATG6 antibody \| ATG6 autophagy related 6 homolog antibody \| Bcl-2-interacting protein beclin antibody \| Beclin 1 (coiled coil moesin like BCL2 interacting
规格或纯度	ExactAb™, Validated, Carrier Free, 1.0mg/mL
生化机理	Plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.
宿主种属	小鼠(Mouse)
特异性	BECN1
种属反应性	人(Human)
免疫原	Recombinant protein of human BECN1 (AA 51-248).
阳性对照	WB: Hela, A431, MCF-7, Raji, Jurkat and SKBR-3 cell lysate; IHC: breast cancer tissues and liver cancer tissues; Flow: Raji cells.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	2A4
Format	Whole IgG
亚型	IgG1
轻链亚型	kappa
SDS-PAGE	150kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 0.05% Sodium azide, pH 7.4.
防腐剂	0.05% Sodium azide
浓度	1.0mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
分子类型	抗体

关联靶点（人）

BECN1 Tbio Beclin-1(Beclin-1) (0 活性数据)

点击查看靶蛋白信息： BECN1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: BECN1 Mouse mAb (Ab090922)

BECN1 Mouse mAb (Ab090922) - Western Blot
All lanes: BECN1 Mouse mAb (Ab090922) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Flow Cytometry: BECN1 Mouse mAb (Ab090922)

BECN1 Mouse mAb (Ab090922) - Flow Cytometry
Flow cytometric analysis of Raji cells using BECN1 Mouse mAb (Ab090922)
and negative control (purple).

IHC: BECN1 Mouse mAb (Ab090922)

BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.

IHC: BECN1 Mouse mAb (Ab090922)

BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.

应用

应用名称	稀释比例
IF/ICC	1/50-1/200
WB	1/500-1/2000
IHC	1/200-1/1000
ELISA	1/10000
Flow Cytometry	1/200-1/400

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F1012037	分析证书	Ab090922
ZJ23F0700750	分析证书	Ab090922
ZJ23F0700751	分析证书	Ab090922

技术文档和文章

流式细胞术

Categories: 技术文章

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流式细胞术

流式细胞仪分析

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前向散射侧向散射二向色滤光片多色流式细胞仪

流式细胞术实验方案

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抗体流式细胞术流式抗体活性染料间接抗体标记

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

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重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

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重组抗体单克隆抗体 IHC 免疫组化抗原修复

利用甲醇通透法进行流式细胞术

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流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

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Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

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Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

可替换产品

引用文献

1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940]
2. Ashkenazi A et al.. (2017) Polyglutamine tracts regulate beclin 1-dependent autophagy.. Nature, 545 (7652): (108-111). [PMID:28445460]
3. Rommens, J M JM and 9 more authors.. (1995) Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21.. Genomics, (10): [PMID:7490091]
4. Liang, X H XH and 7 more authors.. (1998) Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein.. Journal of virology, [PMID:9765397]
5. Aita, V M VM and 8 more authors.. (1999) Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21.. Genomics, (1): [PMID:10395800]
6. Pattingre, Sophie S and 8 more authors.. (2005) Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy.. Cell, (23): [PMID:16179260]
7. Oberstein, Adam A, Jeffrey, Philip D PD and Shi, Yigong Y.. (2007) Crystal structure of the Bcl-XL-Beclin 1 peptide complex: Beclin 1 is a novel BH3-only protein.. The Journal of biological chemistry, (27): [PMID:17337444]
8. Maiuri, M Chiara MC and 12 more authors.. (2007) Functional and physical interaction between Bcl-X(L) and a BH3-like domain in Beclin-1.. The EMBO journal, (16): [PMID:17446862]
9. Feng, Wei W, Huang, Siyi S, Wu, Hao H and Zhang, Mingjie M.. (2007) Molecular basis of Bcl-xL's target recognition versatility revealed by the structure of Bcl-xL in complex with the BH3 domain of Beclin-1.. Journal of molecular biology, (7): [PMID:17659302]
10. Sauermann, M M and 10 more authors.. (2008) Reduced expression of vacuole membrane protein 1 affects the invasion capacity of tumor cells.. Oncogene, (21): [PMID:17724469]
11. Orvedahl, Anthony A and 8 more authors.. (2007) HSV-1 ICP34.5 confers neurovirulence by targeting the Beclin 1 autophagy protein.. Cell host & microbe, (15): [PMID:18005679]
12. Wei, Yongjie Y, Pattingre, Sophie S, Sinha, Sangita S, Bassik, Michael M and Levine, Beth B.. (2008) JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy.. Molecular cell, (20): [PMID:18570871]
13. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976]
14. Sinha, Sangita S, Colbert, Christopher L CL, Becker, Nils N, Wei, Yongjie Y and Levine, Beth B.. (2008) Molecular basis of the regulation of Beclin 1-dependent autophagy by the gamma-herpesvirus 68 Bcl-2 homolog M11.. Autophagy, [PMID:18797192]
15. Itakura, Eisuke E, Kishi, Chieko C, Inoue, Kinji K and Mizushima, Noboru N.. (2008) Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG.. Molecular biology of the cell, [PMID:18843052]
16. Sun, Qiming Q and 5 more authors.. (2008) Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:19050071]
17. Zalckvar, Einat E and 8 more authors.. (2009) DAP-kinase-mediated phosphorylation on the BH3 domain of beclin 1 promotes dissociation of beclin 1 from Bcl-XL and induction of autophagy.. EMBO reports, [PMID:19180116]
18. Matsunaga, Kohichi K and 12 more authors.. (2009) Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages.. Nature cell biology, [PMID:19270696]
19. Oppermann, Felix S FS and 7 more authors.. (2009) Large-scale proteomics analysis of the human kinome.. Molecular & cellular proteomics : MCP, [PMID:19369195]
20. Luo, S S and Rubinsztein, D C DC.. (2010) Apoptosis blocks Beclin 1-dependent autophagosome synthesis: an effect rescued by Bcl-xL.. Cell death and differentiation, [PMID:19713971]
21. Leib, David A DA, Alexander, Diane E DE, Cox, Douglas D, Yin, Jiyi J and Ferguson, Thomas A TA.. (2009) Interaction of ICP34.5 with Beclin 1 modulates herpes simplex virus type 1 pathogenesis through control of CD4+ T-cell responses.. Journal of virology, [PMID:19759141]
22. Sagona, Antonia P AP and 8 more authors.. (2010) PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody.. Nature cell biology, [PMID:20208530]
23. Thoresen, Sigrid B SB, Pedersen, Nina Marie NM, Liestøl, Knut K and Stenmark, Harald H.. (2010) A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates cytokinesis and degradative endocytic traffic.. Experimental cell research, (10): [PMID:20643123]
24. Tang, Daolin D and 10 more authors.. (2010) Endogenous HMGB1 regulates autophagy.. The Journal of cell biology, (6): [PMID:20819940]
25. Strappazzon, Flavie F and 8 more authors.. (2011) Mitochondrial BCL-2 inhibits AMBRA1-induced autophagy.. The EMBO journal, (6): [PMID:21358617]
26. Wirawan, E and 13 more authors.. (2010) Caspase-mediated cleavage of Beclin-1 inactivates Beclin-1-induced autophagy and enhances apoptosis by promoting the release of proapoptotic factors from mitochondria.. Cell death & disease, [PMID:21364619]
27. Li, Hua H and 8 more authors.. (2011) Following cytochrome c release, autophagy is inhibited during chemotherapy-induced apoptosis by caspase 8-mediated cleavage of Beclin 1.. Cancer research, (15): [PMID:21444671]
28. Platta, Harald W HW, Abrahamsen, Hilde H, Thoresen, Sigrid B SB and Stenmark, Harald H.. (2012) Nedd4-dependent lysine-11-linked polyubiquitination of the tumour suppressor Beclin 1.. The Biochemical journal, (1): [PMID:21936852]
29. Liu, Junli J and 19 more authors.. (2011) Beclin1 controls the levels of p53 by regulating the deubiquitination activity of USP10 and USP13.. Cell, (30): [PMID:21962518]
30. Ma, Chunyan C and 5 more authors.. (2012) Receptor signaling lymphocyte-activation molecule family 1 (Slamf1) regulates membrane fusion and NADPH oxidase 2 (NOX2) activity by recruiting a Beclin-1/Vps34/ultraviolet radiation resistance-associated gene (UVRAG) complex.. The Journal of biological chemistry, (25): [PMID:22493499]
31. Robert, Guillaume G and 10 more authors.. (2012) The anti-apoptotic Bcl-B protein inhibits BECN1-dependent autophagic cell death.. Autophagy, [PMID:22498477]
32. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
33. Margariti, Andriana A and 18 more authors.. (2013) XBP1 mRNA splicing triggers an autophagic response in endothelial cells through BECLIN-1 transcriptional activation.. The Journal of biological chemistry, (11): [PMID:23184933]
34. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
35. Fogel, Adam I AI and 8 more authors.. (2013) Role of membrane association and Atg14-dependent phosphorylation in beclin-1-mediated autophagy.. Molecular and cellular biology, [PMID:23878393]
36. Xia, Pengyan P and 15 more authors.. (2013) WASH inhibits autophagy through suppression of Beclin 1 ubiquitination.. The EMBO journal, (16): [PMID:23974797]
37. Seto, Shintaro S and 5 more authors.. (2013) Rab39a interacts with phosphatidylinositol 3-kinase and negatively regulates autophagy induced by lipopolysaccharide stimulation in macrophages.. PloS one, [PMID:24349490]
38. Su, Minfei M and 5 more authors.. (2014) Targeting γ-herpesvirus 68 Bcl-2-mediated down-regulation of autophagy.. The Journal of biological chemistry, (21): [PMID:24443581]
39. Mandell, Michael A MA and 13 more authors.. (2014) TRIM proteins regulate autophagy and can target autophagic substrates by direct recognition.. Developmental cell, (25): [PMID:25127057]
40. McKnight, Nicole C NC and 9 more authors.. (2014) Beclin 1 is required for neuron viability and regulates endosome pathways via the UVRAG-VPS34 complex.. PLoS genetics, [PMID:25275521]
41. Baskaran, Sulochanadevi S and 8 more authors.. (2014) Architecture and dynamics of the autophagic phosphatidylinositol 3-kinase complex.. eLife, (9): [PMID:25490155]
42. Cianfanelli, Valentina V, D'Orazio, Melania M and Cecconi, Francesco F.. (2015) AMBRA1 and BECLIN 1 interplay in the crosstalk between autophagy and cell proliferation.. Cell cycle (Georgetown, Tex.), [PMID:25803737]
43. Chauhan, Santosh S, Mandell, Michael A MA and Deretic, Vojo V.. (2015) IRGM governs the core autophagy machinery to conduct antimicrobial defense.. Molecular cell, (7): [PMID:25891078]
44. Siddiqui, Mohammad Adnan MA, Mukherjee, Sushovita S, Manivannan, Praveen P and Malathi, Krishnamurthy K.. (2015) RNase L Cleavage Products Promote Switch from Autophagy to Apoptosis by Caspase-Mediated Cleavage of Beclin-1.. International journal of molecular sciences, (31): [PMID:26263979]
45. Kimura, Tomonori T and 6 more authors.. (2015) TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity.. The Journal of cell biology, (14): [PMID:26347139]
46. Liu, Kai K and 12 more authors.. (2016) Negative regulation of phosphatidylinositol 3-phosphate levels in early-to-late endosome conversion.. The Journal of cell biology, (18): [PMID:26783301]
47. Mandell, Michael A MA and 8 more authors.. (2016) TRIM17 contributes to autophagy of midbodies while actively sparing other targets from degradation.. Journal of cell science, (1): [PMID:27562068]
48. Chauhan, Santosh S and 11 more authors.. (2016) TRIMs and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-direct Autophagy in Endomembrane Damage Homeostasis.. Developmental cell, (10): [PMID:27693506]
49. Tian, Miao M and 5 more authors.. (2017) Beclin1 antagonizes LAPTM4B-mediated EGFR overactivation in gastric cancer cells.. Gene, (30): [PMID:28479384]
50. Fusco, Carmela C and 13 more authors.. (2018) TRIM50 regulates Beclin 1 proautophagic activity.. Biochimica et biophysica acta. Molecular cell research, [PMID:29604308]
51. Di Rienzo, M M and 22 more authors.. (2019) Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains.. Science advances, [PMID:31123703]
52. Miao, Guangyan; Zhang, Yujie; Chen, Di and Zhang, Hong.. (2020) The ER-Localized Transmembrane Protein TMEM39A/SUSR2 Regulates Autophagy by Controlling the Trafficking of the PtdIns(4)P Phosphatase SAC1.. Molecular cell, (6): [PMID:31806350]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

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体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

BECN1 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器