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Recombinant ACTN4 Antibody

    应用:
  • IF/ICC
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab088349-10μl
10μl 现货 Stock Image
Ab088349-50μl
50μL 现货 Stock Image
Ab088349-100μl
100μL 现货 Stock Image
Ab088349-1ml
1ml 期货 Stock Image

基本信息

产品名称 Recombinant ACTN4 Antibody
别名 α-辅肌动蛋白4抗体 | 重组 ACTN4 抗体
英文别名 alpha Actinin 4 antibody | actinin 4 antibody | Actinin alpha 4 antibody | actinin4 antibody | ACTN 4 antibody | ACTN4 antibody | ACTN4_HUMAN antibody | Alpha-actinin-4 antibody | DKFZp686K23158 antibody | F actin cross linking proteinantibody | F-actin c
规格或纯度 ExactAb™, Validated, 重组, 0.5mg/mL
宿主种属 兔(Rabbit)
特异性 ACTN4
应用 WB,IF,ICC
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
免疫原 A synthetic peptide derived from human alpha Actinin 4 (AA 1-14).
阳性对照 WB: C6, NIH/3T3, HeLa, A549, K562, A431 lysates. ICC/IF: HeLa cells.
偶联 Unconjugated

产品属性

抗体类型 Primary antibody
克隆类型 重组抗体
Format Whole IgG
亚型 IgG
SDS-PAGE 150kDa
纯化方法 Antigen affinity purification
来源 Cell supernatant
物理外观 Liquid
储存缓冲液 50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂 0.01% Sodium azide
浓度 0.5mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

ACTN4 Tbio α-辅肌动蛋白-4(Alpha-actinin-4) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

Recombinant ACTN4 Antibody (Ab088349) - Western Blot
All lanes: Recombinant ACTN4 Antibody (Ab088349) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 105 kDa
Observed band size: 103 kDa

Recombinant ACTN4 Antibody (Ab088349) - Western Blot
All lanes: Recombinant ACTN4 Antibody (Ab088349) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 105 kDa
Observed band size: 103 kDa

Recombinant ACTN4 Antibody (Ab088349) - IF
IF analysis of ACTN4 (green) in HeLa cells using Recombinant ACTN4 Antibody (Ab088349) and cells were counterstained with DAPI (blue).

应用

应用名称 稀释比例
WB

1/500-1/1000

IF/ICC

1/50-1/200

质检证书(CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F0700746 分析证书 Ab088349
ZJ23F0700747 分析证书 Ab088349
ZJ24F1011933 分析证书 Ab088349

技术文档和文章

如何选择和使用抗体 — 一抗
How to choose and use antibodies-primary antibody?
How to choose and use antibodies-primary antibody?
How to choose and use antibodies-primary antibody?
组织块整体免疫组织化学染色 实验方案和技巧
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
Protocol and tips for whole mount immunohistochemical staining
How to choose and use antibodies-primary antibody?

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