ACE2 Mouse mAb

COA

级别和纯度:

无载体

已验证

ExactAb™

1.0 mg/mL

应用:

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: K92008_4B6
偶联: Unconjugated

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab087119-10μl	10μl	现货
Ab087119-50μl	50μL	现货
Ab087119-100μl	100μL	现货
Ab087119-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: ACE2

Accession#: Q9BYF1 (2) ACE2 (3) Gene ID: 59272 (2)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本信息

产品名称	ACE2 Mouse mAb
别名	血管紧张素转换酶2抗体 \| 血管紧张素转换酶2单克隆抗体 \| ACE2抗体 \| ACE2单克隆抗体
英文别名	3.4.17.- \| ACEH \| ACE-related carboxypeptidase \| Angiotensin-converting enzyme homolog \| Angiotensin-converting enzyme-related carboxypeptidase \| EC 3.4.17.23 \| Metalloprotease MPROT15 \| ACE 2 antibody \| ACE related carboxypeptidase antibody \| ACE-related
规格或纯度	ExactAb™, Validated, Carrier Free, 1.0 mg/mL
生化机理	Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
宿主种属	小鼠(Mouse)
特异性	ACE2
种属反应性	人(Human)
免疫原	Recombinant human ACE2 (AA 21-160).
阳性对照	WB: Calu-3 lysates. IHC: Human kidney tissue.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	K92008_4B6
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	150mM PBS, 0.09% Sodium azide, pH 7.4
防腐剂	0.09% Sodium azide
浓度	1.0 mg/mL
储存温度	-80℃储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	短期储存2-8℃（3个月）；长期储存-20℃（12个月）；储存-80℃（36个月）；收货后建议分装，避免反复冻融。
分子类型	抗体

关联靶点（人）

ACE2 Tchem 血管紧张素转换酶2(Angiotensin-converting enzyme 2) (0 活性数据)

点击查看靶蛋白信息： ACE2

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: ACE2 Mouse mAb (Ab087119)

ACE2 Mouse mAb (Ab087119) - Western Blot
15 µg of Calu-3 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. ACE2 Mouse mAb (Ab087119) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. ACE2 band was visualized using ECL Western Blotting Substrate. Result: ACE2 Mouse mAb (Ab087119) can detect ACE2 by Western blotting.

IHC: ACE2 Mouse mAb (Ab087119)

ACE2 Mouse mAb (Ab087119) - IHC
IHC analysis of kidney tissue by ACE2 Mouse mAb (Ab087119).
IHC was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with ACE2 Mouse mAb (Ab087119) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Microvillus of cells in tubules are positively stained.

应用

应用名称	稀释比例
WB	1/1000
IHC	1/200

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0420811	分析证书	Ab087119
ZJ25F0420810	分析证书	Ab087119
ZJ25F0420812	分析证书	Ab087119

引用文献

1. Vickers C, Hales P, Kaushik V, Dick L, Gavin J, Tang J, Godbout K, Parsons T, Baronas E, Hsieh F et al.. (2002) Hydrolysis of biological peptides by human angiotensin-converting enzyme-related carboxypeptidase.. J Biol Chem, 277 (17): (14838-43). [PMID:11815627]
2. Donoghue M, Hsieh F, Baronas E, Godbout K, Gosselin M, Stagliano N, Donovan M, Woolf B, Robison K, Jeyaseelan R et al.. (2000) A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9.. Circ Res, 87 (5): (E1-9). [PMID:10969042]
3. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al.. (2003) The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.. Genome Res, 13 (10): (2265-70). [PMID:12975309]
4. Hernández Prada JA, Ferreira AJ, Katovich MJ, Shenoy V, Qi Y, Santos RA, Castellano RK, Lampkins AJ, Gubala V, Ostrov DA et al.. (2008) Structure-based identification of small-molecule angiotensin-converting enzyme 2 activators as novel antihypertensive agents.. Hypertension, 51 (5): (1312-7). [PMID:18391097]
5. Deaton DN, Gao EN, Graham KP, Gross JW, Miller AB, Strelow JM. (2008) Thiol-based angiotensin-converting enzyme 2 inhibitors: P1 modifications for the exploration of the S1 subsite.. Bioorg Med Chem Lett, 18 (2): (732-7). [PMID:18078750]
6. Li F, Li W, Farzan M, Harrison SC. (2005) Structure of SARS coronavirus spike receptor-binding domain complexed with receptor.. Science, 309 (5742): (1864-8). [PMID:16166518]
7. Hoffmann M, Kleine-Weber H, Schroeder S, Krüger N, Herrler T, Erichsen S, Schiergens TS, Herrler G, Wu NH, Nitsche A et al.. (2020) SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor.. Cell, 181 (2): (271-280.e8). [PMID:32142651]
8. Walls AC, Park YJ, Tortorici MA, Wall A, McGuire AT, Veesler D. (2020) Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein.. Cell, 181 (2): (281-292.e6). [PMID:32155444]
9. Song W, Gui M, Wang X, Xiang Y. (2018) Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2.. PLoS Pathog, 14 (8): (e1007236). [PMID:30102747]
10. Davies J, Randeva HS, Chatha K, Hall M, Spandidos DA, Karteris E, Kyrou I. (2020) Neuropilin‑1 as a new potential SARS‑CoV‑2 infection mediator implicated in the neurologic features and central nervous system involvement of COVID‑19.. Mol Med Rep, 22 (5): (4221-4226). [PMID:33000221]
11. Daly JL, Simonetti B, Klein K, Chen KE, Williamson MK, Antón-Plágaro C, Shoemark DK, Simón-Gracia L, Bauer M, Hollandi R et al.. (2020) Neuropilin-1 is a host factor for SARS-CoV-2 infection.. Science, 370 (6518): (861-865). [PMID:33082294]
12. Olaleye OA, Kaur M, Onyenaka CC. (2020) Ambroxol Hydrochloride Inhibits the Interaction between Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein's Receptor Binding Domain and Recombinant Human ACE2.. bioRxiv, [PMID:32995775] [10.1101/2020.09.13.295691]
13. Deaton DN, Graham KP, Gross JW, Miller AB.. (2008) Thiol-based angiotensin-converting enzyme 2 inhibitors: P1' modifications for the exploration of the S1' subsite.. Bioorg Med Chem Lett, 18 (5): (1681-1687). [PMID:18243695] [10.1016/j.bmcl.2008.01.046]
14. Charton J, Gauriot M, Guo Q, Hennuyer N, Marechal X, Dumont J, Hamdane M, Pottiez V, Landry V, Sperandio O, Flipo M, Buee L, Staels B, Leroux F, Tang WJ, Deprez B, Deprez-Poulain R.. (2014) Imidazole-derived 2-[N-carbamoylmethyl-alkylamino]acetic acids, substrate-dependent modulators of insulin-degrading enzyme in amyloid-β hydrolysis.. Eur J Med Chem, 79 (184-193). [PMID:24735644] [10.1016/j.ejmech.2014.04.009]
15. Choi JY, Fuerst R, Knapinska AM, Taylor AB, Smith L, Cao X, Hart PJ, Fields GB, Roush WR.. (2017) Structure-Based Design and Synthesis of Potent and Selective Matrix Metalloproteinase 13 Inhibitors.. J Med Chem, 60 (13): (5816-5825). [PMID:28653849] [10.1021/acs.jmedchem.7b00514]
16. Tipnis, S R SR and 5 more authors.. (2000) A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase.. The Journal of biological chemistry, (27): [PMID:10924499]
17. Harmer, Dan D, Gilbert, Maureen M, Borman, Richard R and Clark, Kenneth L KL.. (2002) Quantitative mRNA expression profiling of ACE 2, a novel homologue of angiotensin converting enzyme.. FEBS letters, (4): [PMID:12459472]
18. Zisman, Lawrence S LS and 6 more authors.. (2003) Increased angiotensin-(1-7)-forming activity in failing human heart ventricles: evidence for upregulation of the angiotensin-converting enzyme Homologue ACE2.. Circulation, (7): [PMID:14504186]
19. Li, Wenhui W and 11 more authors.. (2003) Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus.. Nature, (27): [PMID:14647384]
20. Towler, Paul P and 11 more authors.. (2004) ACE2 X-ray structures reveal a large hinge-bending motion important for inhibitor binding and catalysis.. The Journal of biological chemistry, (23): [PMID:14754895]
21. Kristiansen, Troels Zakarias TZ and 8 more authors.. (2004) A proteomic analysis of human bile.. Molecular & cellular proteomics : MCP, [PMID:15084671]
22. Hamming, I I and 5 more authors.. (2004) Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis.. The Journal of pathology, [PMID:15141377]
23. Goulter, Andrew B AB, Goddard, Martin J MJ, Allen, Jennifer C JC and Clark, Kenneth L KL.. (2004) ACE2 gene expression is up-regulated in the human failing heart.. BMC medicine, (19): [PMID:15151696]
24. Douglas, Gabrielle C GC and 6 more authors.. (2004) The novel angiotensin-converting enzyme (ACE) homolog, ACE2, is selectively expressed by adult Leydig cells of the testis.. Endocrinology, [PMID:15231706]
25. Lin, Qishan Q, Keller, Rebecca S RS, Weaver, Barbara B and Zisman, Lawrence S LS.. (2004) Interaction of ACE2 and integrin beta1 in failing human heart.. Biochimica et biophysica acta, (4): [PMID:15276642]
26. Li, Wenhui W and 7 more authors.. (2004) Efficient replication of severe acute respiratory syndrome coronavirus in mouse cells is limited by murine angiotensin-converting enzyme 2.. Journal of virology, [PMID:15452268]
27. Huentelman, Matthew J MJ and 6 more authors.. (2004) Structure-based discovery of a novel angiotensin-converting enzyme 2 inhibitor.. Hypertension (Dallas, Tex. : 1979), [PMID:15492138]
28. Burrell, Louise M LM and 11 more authors.. (2005) Myocardial infarction increases ACE2 expression in rat and humans.. European heart journal, [PMID:15671045]
29. Li, Wenhui W and 15 more authors.. (2005) Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2.. The EMBO journal, (20): [PMID:15791205]
30. Hofmann, Heike H and 5 more authors.. (2005) Human coronavirus NL63 employs the severe acute respiratory syndrome coronavirus receptor for cellular entry.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:15897467]
31. Itoyama, Satoru S and 13 more authors.. (2005) Identification of an alternative 5'-untranslated exon and new polymorphisms of angiotensin-converting enzyme 2 gene: lack of association with SARS in the Vietnamese population.. American journal of medical genetics. Part A, (1): [PMID:15937940]
32. Lambert, Daniel W DW and 7 more authors.. (2005) Tumor necrosis factor-alpha convertase (ADAM17) mediates regulated ectodomain shedding of the severe-acute respiratory syndrome-coronavirus (SARS-CoV) receptor, angiotensin-converting enzyme-2 (ACE2).. The Journal of biological chemistry, (26): [PMID:15983030]
33. Guy, Jodie L JL, Jackson, Richard M RM, Jensen, Hanne A HA, Hooper, Nigel M NM and Turner, Anthony J AJ.. (2005) Identification of critical active-site residues in angiotensin-converting enzyme-2 (ACE2) by site-directed mutagenesis.. The FEBS journal, [PMID:16008552]
34. Vincent, Martin J MJ and 7 more authors.. (2005) Chloroquine is a potent inhibitor of SARS coronavirus infection and spread.. Virology journal, (22): [PMID:16115318]
35. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005]
36. Kowalczuk, Sonja S and 5 more authors.. (2008) A protein complex in the brush-border membrane explains a Hartnup disorder allele.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:18424768]
37. Rushworth, Christopher A CA, Guy, Jodie L JL and Turner, Anthony J AJ.. (2008) Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis.. The FEBS journal, [PMID:19021774]
38. Camargo, Simone M R SM and 11 more authors.. (2009) Tissue-specific amino acid transporter partners ACE2 and collectrin differentially interact with hartnup mutations.. Gastroenterology, [PMID:19185582]
39. Wu, Kailang K, Li, Weikai W, Peng, Guiqing G and Li, Fang F.. (2009) Crystal structure of NL63 respiratory coronavirus receptor-binding domain complexed with its human receptor.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:19901337]
40. Shulla, Ana A and 5 more authors.. (2011) A transmembrane serine protease is linked to the severe acute respiratory syndrome coronavirus receptor and activates virus entry.. Journal of virology, [PMID:21068237]
41. Heurich, Adeline A and 5 more authors.. (2014) TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.. Journal of virology, [PMID:24227843]
42. Wang, Wang W and 10 more authors.. (2016) Angiotensin-Converting Enzyme 2 Metabolizes and Partially Inactivates Pyr-Apelin-13 and Apelin-17: Physiological Effects in the Cardiovascular System.. Hypertension (Dallas, Tex. : 1979), [PMID:27217402]
43. Yang, Peiran P and 9 more authors.. (2017) [Pyr1]Apelin-13(1-12) Is a Biologically Active ACE2 Metabolite of the Endogenous Cardiovascular Peptide [Pyr1]Apelin-13.. Frontiers in neuroscience, [PMID:28293165]
44. Wrapp, Daniel and 7 more authors.. (2020) Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation.. Science (New York, N.Y.), (13): [PMID:32075877]
45. Zou, Xin and 5 more authors.. (2020) Single-cell RNA-seq data analysis on the receptor ACE2 expression reveals the potential risk of different human organs vulnerable to 2019-nCoV infection.. Frontiers of medicine, [PMID:32170560]
46. Ou, Xiuyuan and 17 more authors.. (2020) Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV.. Nature communications, (27): [PMID:32221306]
47. Shang, Jian and 8 more authors.. (2020) Structural basis of receptor recognition by SARS-CoV-2.. Nature, [PMID:32225175]
48. Jackson, Daniel J and 15 more authors.. (2020) Association of respiratory allergy, asthma, and expression of the SARS-CoV-2 receptor ACE2.. The Journal of allergy and clinical immunology, [PMID:32333915]
49. Lamers, Mart M and 20 more authors.. (2020) SARS-CoV-2 productively infects human gut enterocytes.. Science (New York, N.Y.), (3): [PMID:32358202]
50. Zang, Ruochen and 13 more authors.. (2020) TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes.. Science immunology, (13): [PMID:32404436]
51. Ziegler, Carly G K and 66 more authors.. (2020) SARS-CoV-2 Receptor ACE2 Is an Interferon-Stimulated Gene in Human Airway Epithelial Cells and Is Detected in Specific Cell Subsets across Tissues.. Cell, (28): [PMID:32413319]
52. Smith, Joan C and 6 more authors.. (2020) Cigarette Smoke Exposure and Inflammatory Signaling Increase the Expression of the SARS-CoV-2 Receptor ACE2 in the Respiratory Tract.. Developmental cell, (8): [PMID:32425701]
53. Hikmet, Feria and 5 more authors.. (2020) The protein expression profile of ACE2 in human tissues.. Molecular systems biology, [PMID:32715618]
54. and Alkotaji, Myasar.. (2020) Azithromycin and ambroxol as potential pharmacotherapy for SARS-CoV-2.. International journal of antimicrobial agents, [PMID:33045350]
55. Beddingfield, Brandon J and 7 more authors.. (2021) The Integrin Binding Peptide, ATN-161, as a Novel Therapy for SARS-CoV-2 Infection.. JACC. Basic to translational science, [PMID:33102950]
56. Shilts, Jarrod; Crozier, Thomas W M; Greenwood, Edward J D; Lehner, Paul J and Wright, Gavin J.. (2021) No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor.. Scientific reports, (11): [PMID:33432067]
57. Blume, Cornelia and 26 more authors.. (2021) A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection.. Nature genetics, [PMID:33432184]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

ACE2 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器