货号 (SKU)

包装规格

是否现货

价格

数量

Ab008407-10μg

10μg

现货

Ab008407-50μg

50μg

现货

Ab008407-100μg

100μg

现货

Ab008407-1mg

1mg

期货

基本信息

产品名称	S100B Mouse mAb
别名	S100B小鼠单克隆抗体 \| S100B小鼠单抗
英文别名	S100 calcium-binding protein B \| S-100 protein beta chain \| S-100 protein subunit beta
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	S100B
种属反应性	人(Human)
免疫原	Recombinant Human S100B Protein, C-His tag
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	22G7-5
Format	Whole IgG
亚型	Mouse IgG1
SDS-PAGE	25.6 kDa (Light Chain) & 52.1 kDa (Heavy Chain), under reducing conditions; 174.5 kDa, under non-reducing conditions.
纯化方法	Affinity chromatography
配对抗体	[Ab008408] Monoclonal S100B Antibody as Detection or Capture antibody in ELISA, CLIA, LF, GICA, FIA
物理外观	Purified polyclonal antibodies Antibody
储存缓冲液	10mM PBS, pH7.4
防腐剂	No
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	37°C 下短期保存（7 天） 4°C 下短期保存（30 天）。在 -20±5°C 下长期储存（36 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

S100B Tchem 蛋白质S100-B(Protein S100-B) (0 活性数据)

点击查看靶蛋白信息： S100B

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Sandwich ELISA - Human S100B Antibody Pair (Ab008408 & Ab008407)

Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008408) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 30.3 pg/mL
Range: 0.1 ng/mL - 3.2 ng/mL

Sandwich ELISA - Human S100B Antibody Pair (Ab008407 & Ab008408)

Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008407) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 18.8 pg/mL
Range: 0.13 ng/mL - 3.1 ng/mL

SDS-PAGE: S100B Mouse mAb (Ab008407),

S100B Mouse mAb (Ab008407) - SDS-PAGE
3 μg/lane of S100B Mouse mAb (Ab008407) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 26.0 kDa (Light Chain) & 54.2 kDa (Heavy Chain) under reducing conditions and 178.7 kDa under non-reducing conditions.

Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA

Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA
·Capture antibody: S100B Mouse mAb (Ab008408)
·Detection antibody: S100B Mouse mAb (Ab008407)

应用

应用名称	稀释比例
CLIA	Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
FLFIA	Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL

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C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F1011990	分析证书	Ab008407
ZJ23F0901142	分析证书	Ab008407

可替换产品

引用文献

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334]
2. Markowitz J, Chen I, Gitti R, Baldisseri DM, Pan Y, Udan R, Carrier F, MacKerell AD, Weber DJ.. (2004) Identification and characterization of small molecule inhibitors of the calcium-dependent S100B-p53 tumor suppressor interaction.. J Med Chem, 47 (21): (5085-5093). [PMID:15456252] [10.1021/jm0497038]
3. Yoshimura C, Miyafusa T, Tsumoto K.. (2013) Identification of small-molecule inhibitors of the human S100B-p53 interaction and evaluation of their activity in human melanoma cells.. Bioorg Med Chem, 21 (5): (1109-1115). [PMID:23375094] [10.1016/j.bmc.2012.12.042]
4. Allore, R J RJ and 6 more authors.. (1990) Cloning and expression of the human S100 beta gene.. The Journal of biological chemistry, (15): [PMID:2394738]
5. Jensen, R R, Marshak, D R DR, Anderson, C C, Lukas, T J TJ and Watterson, D M DM.. (1985) Characterization of human brain S100 protein fraction: amino acid sequence of S100 beta.. Journal of neurochemistry, [PMID:4031854]
6. Baudier, J J, Glasser, N N, Haglid, K K and Gerard, D D.. (1984) Purification, characterization and ion binding properties of human brain S100b protein.. Biochimica et biophysica acta, (23): [PMID:6487634]
7. Smith, S P SP and Shaw, G S GS.. (1998) A novel calcium-sensitive switch revealed by the structure of human S100B in the calcium-bound form.. Structure (London, England : 1993), (15): [PMID:9519411]
8. Yang, Q Q, O'Hanlon, D D, Heizmann, C W CW and Marks, A A.. (1999) Demonstration of heterodimer formation between S100B and S100A6 in the yeast two-hybrid system and human melanoma.. Experimental cell research, (1): [PMID:9925766]
9. Hattori, M M and 64 more authors.. (2000) The DNA sequence of human chromosome 21.. Nature, (18): [PMID:10830953]
10. McClintock, Kimberly A KA and Shaw, Gary S GS.. (2003) A novel S100 target conformation is revealed by the solution structure of the Ca2+-S100B-TRTK-12 complex.. The Journal of biological chemistry, (21): [PMID:12480931]
11. Lee, Tih-Shih TS and 12 more authors.. Gene expression in temporal lobe epilepsy is consistent with increased release of glutamate by astrocytes.. Molecular medicine (Cambridge, Mass.), [PMID:17515952]
12. Marlatt, Nicole M NM and Shaw, Gary S GS.. (2007) Amide exchange shows calcium-induced conformational changes are transmitted to the dimer interface of S100B.. Biochemistry, (26): [PMID:17536784]
13. Liang, Jie J and 15 more authors.. (2007) Differential expression of calcium-related genes in gastric cancer cells transfected with cellular prion protein.. Biochemistry and cell biology = Biochimie et biologie cellulaire, [PMID:17612632]
14. Friel, Lara A LA and 9 more authors.. (2007) The calcium binding protein, S100B, is increased in the amniotic fluid of women with intra-amniotic infection/inflammation and preterm labor with intact or ruptured membranes.. Journal of perinatal medicine, [PMID:17624933]
15. Gilquin, Benoît B and 11 more authors.. (2010) The calcium-dependent interaction between S100B and the mitochondrial AAA ATPase ATAD3A and the role of this complex in the cytoplasmic processing of ATAD3A.. Molecular and cellular biology, [PMID:20351179]
16. Park, Hajeung H, Adsit, Floyd G FG and Boyington, Jeffrey C JC.. (2010) The 1.5 Å crystal structure of human receptor for advanced glycation endproducts (RAGE) ectodomains reveals unique features determining ligand binding.. The Journal of biological chemistry, (24): [PMID:20943659]
17. Ostendorp, Thorsten T, Diez, Joachim J, Heizmann, Claus W CW and Fritz, Günter G.. (2011) The crystal structures of human S100B in the zinc- and calcium-loaded state at three pH values reveal zinc ligand swapping.. Biochimica et biophysica acta, [PMID:20950652]
18. Yamaguchi, Fuminori F and 6 more authors.. (2012) S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation.. The Journal of biological chemistry, (20): [PMID:22399290]

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体积（添加到小瓶中）

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所需的复溶浓度

S100B Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器

S100B Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器