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Vimentin Mouse mAb - Primary antibody, specific to Vimentin, IgG2b

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2b
  • Clone Number: AB07/4H7
  • Conjugation: Unconjugated
In stock
Item Number
Ab179444
Grouped product items
SKU Size
Availability
 Price Qty
Ab179444-10μl
10μl
≥10
$66.90
Ab179444-50μl
50μl
10
$199.90
Ab179444-100μl
100μl
7
$299.90
Ab179444-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,499.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (AB07/4H7); Mouse anti Vimentin Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated

View related series
Click to view target information: VIM
Accession#:P08670 (5) Flow Cytometry (1158) Gene ID:7431 (5) VIM (5) Vimentin (5)

Basic Description

Product Name Vimentin Mouse mAb - Primary antibody, specific to Vimentin, IgG2b
Synonyms CTRCT30 antibody | Epididymis luminal protein 113 antibody | FLJ36605 antibody | HEL113 antibody | VIM antibody | VIME_HUMAN antibody | Vimentin antibody | Anti-Vimentin Antibody | Vimentin Monoclonal Antibody | Anti-Human VIM/Vimentin Antibody | Mouse an
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
Host species Mouse
Specificity Vimentin
Immunogen Recombinant full length protein corresponding to Human Vimentin
Positive Control WB: HeLa, HEK293, K562, A549, Jurkat, SK-N-SH and HUVEC whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1/2,000-1/10,000 ; Predicted molecular weight: 54 kDa
Flow: 0.008 μg/mL – 1 μg/mL
ICC/IF: 2 μg/mL

Product Properties

Isotype IgG2b
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

VIM Tbio Vimentin (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 53.7 kDa
Observed band size: 55.4 kDa
Exposure time: 25.0 s 

Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 53.7 kDa
Observed band size: 55.5 kDa
Exposure time: 25.0 s 

Vimentin Mouse mAb (Ab179444) - Western Blot
All lanes: Vimentin Antibody (Ab179444) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 53.7 kDa
Observed band size: 55.4 kDa
Exposure time: 10.1 s 

Vimentin Mouse mAb (Ab179444) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab179444) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Vimentin Mouse mAb (Ab179444) - ICC/IF
IF analysis of Vimentin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vimentin Mouse mAb (Ab179444) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

2 μg/mL
Flow Cytometry

0.008-1μg/mL

WB

1/2,000-1/10,000 ; Predicted molecular weight: 54 kDa

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24R0400481 Certificate of Analysis Apr 26, 2024 Ab179444
ZJ24F0102679 Certificate of Analysis Feb 01, 2024 Ab179444
ZJ24F0102678 Certificate of Analysis Feb 01, 2024 Ab179444
ZJ24F0102677 Certificate of Analysis Feb 01, 2024 Ab179444

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