Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab156188-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$66.90
|
|
|
Ab156188-50μl
|
50μl |
8
|
$199.90
|
|
|
Ab156188-100μl
|
100μl |
7
|
$299.90
|
|
|
Ab156188-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,499.90
|
|
mAb (AB10/4F11); Mouse anti Vimentin Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
| Product Name | Vimentin Mouse mAb - Primary antibody, specific to Vimentin, IgG2a |
|---|---|
| Synonyms | CTRCT30 antibody | Epididymis luminal protein 113 antibody | FLJ36605 antibody | HEL113 antibody | VIM antibody | VIME_HUMAN antibody | Vimentin antibody | Anti-Vimentin Antibody | Vimentin Monoclonal Antibody | Anti-Human VIM/Vimentin Antibody | Mouse an |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | Vimentin |
| Immunogen | Recombinant full length protein corresponding to human Vimentin |
| Positive Control | WB: HeLa, HEK293, A549, Jurkat and NIH/3T3 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Application |
| Isotype | IgG2a |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C for short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Vimentin Mouse mAb (Ab156188) - Western Blot
All lanes: Vimentin Antibody (Ab156188) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53.7 kDa
Observed band size: 54.9 kDa
Exposure time: 26.9 s
Vimentin Mouse mAb (Ab156188) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vimentin Mouse mAb (Ab156188) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control.
Vimentin Mouse mAb (Ab156188) - ICC/IF
IF analysis of Vimentin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vimentin Mouse mAb (Ab156188) at 2 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| Flow Cytometry |
0.016-2.0μg/mL
|
| IF/ICC | 2 μg/mL |
| WB | 1/2,000-1/20,000 ; Predicted molecular weight: 54 kDa |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Nov 16, 2023 | Ab156188 | |
| Certificate of Analysis | Nov 16, 2023 | Ab156188 |
Starting at $66.90
Starting at $66.90
Starting at $59.90