Igepal CA-210 | CAS_118-96-7 | PDSP1_001087 | Ethanol,1,3,3-tetramethylbutyl)phenoxy]- | 2-(4-(2,4,4-Trimethylpentan-2-yl)phenoxy)ethan-1-ol | Octoxynol-1 | Triton X 305 | P-TERT-OCTYLPHENYL (2-HYDROXYETHYL)ETHER | AT25387 | Igepal CA 630 | FT-0673247 | T
Specifications & Purity
for molecular biology
Biochemical and Physiological Mechanisms
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Storage Temp
Room temperature
Shipped In
Normal
Grade
for molecular biology
Product Description
General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection
Application
Triton™ X-100 has been used:
• In immunohistochemistry for staining the Flat-mount retinas
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
• To permeabilise cells during Immunofluorescent microscopic studies
• As a positive control in LDH assay to determine the cell membrane integrity
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
• For the preparation of outer membrane protein exctract
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells
• In the treatment of tissue sections for Immunofluorescence labeling
This compound belongs to the class of organic compounds known as phenylpropanes. These are organic compounds containing a phenylpropane moiety.
External Descriptors
a small molecule
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
605 Citations
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