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SIL1 Mouse mAb - Primary antibody, specific to SIL1

    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: OTI3E3
  • Conjugation: Unconjugated
In stock
Item Number
Ab127610
Grouped product items
SKU Size
Availability
Price Qty
Ab127610-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab127610-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$269.90
Ab127610-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90
Ab127610-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

mAb (OTI3E3); Mouse anti Human SIL1 Antibody; WB, IHC, IF/ICC, Flow Cytometry; Unconjugated

Basic Description

Product Name SIL1 Mouse mAb - Primary antibody, specific to SIL1
Synonyms BAP | BiP associated protein | BiP-associated protein | Endoplasmic reticulum chaperone SIL 1 | Endoplasmic reticulum chaperone SIL1 | MSS | Nucleotide exchange factor SIL 1 | Nucleotide exchange factor SIL1 | SIL 1 | sil1 | SIL1 homolog | SIL1 homolog en
Specifications & Purity ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
Host species Mouse
Specificity SIL1
Immunogen Recombinant protein of human SIL1.
Positive Control WB: Hep G2, HeLa, A549 and A431 cell lysates. IHC: Human kidney, liver, carcinoma of liver, adenocarcinoma of ovary tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY SIL1, HT29 cells. Flow Cytometry: HEK293T transfected with pCMV6-ENTRY SIL1.
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Mouse anti Human SIL1 Antibody, Monoclonal (OTI3E3), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Product Properties

Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

SIL1 Tbio Nucleotide exchange factor SIL1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

SIL1 Mouse mAb (Ab127610) - Western Blot
All lanes: SIL1 Mouse mAb (Ab127610) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 48, 54 kDa
The expression profile and molecular weight observed, which is due to glycosylation.
Exposure time: 57.2 s

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab127610, Dilution 1/50)

SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using SIL1 Mouse mAb (Ab127610). (Heatinduced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)

SIL1 Mouse mAb (Ab127610) - IF/ICC
SIL1 Mouse mAb (Ab127610) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.

SIL1 Mouse mAb (Ab127610) - IF/ICC
Immunofluorescent staining of HT29 cells using SIL1 Mouse mAb (Ab127610).

SIL1 Mouse mAb (Ab127610) - Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY SIL1 (Red) or empty vector control plasmid (Blue) were immunostained with SIL1 Mouse mAb (Ab127610), and then analyzed by flow cytometry.

Application

Application Dilution info
IHC

1/100-1/500

IF/ICC

1/100

WB

1/200-1/1000

Flow Cytometry

1mg/ml

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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