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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab127610-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$89.90
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Ab127610-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$269.90
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Ab127610-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$379.90
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Ab127610-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
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mAb (OTI3E3); Mouse anti Human SIL1 Antibody; WB, IHC, IF/ICC, Flow Cytometry; Unconjugated
| Product Name | SIL1 Mouse mAb - Primary antibody, specific to SIL1 |
|---|---|
| Synonyms | BAP | BiP associated protein | BiP-associated protein | Endoplasmic reticulum chaperone SIL 1 | Endoplasmic reticulum chaperone SIL1 | MSS | Nucleotide exchange factor SIL 1 | Nucleotide exchange factor SIL1 | SIL 1 | sil1 | SIL1 homolog | SIL1 homolog en |
| Specifications & Purity | ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5. |
| Host species | Mouse |
| Specificity | SIL1 |
| Immunogen | Recombinant protein of human SIL1. |
| Positive Control | WB: Hep G2, HeLa, A549 and A431 cell lysates. IHC: Human kidney, liver, carcinoma of liver, adenocarcinoma of ovary tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY SIL1, HT29 cells. Flow Cytometry: HEK293T transfected with pCMV6-ENTRY SIL1. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Human SIL1 Antibody, Monoclonal (OTI3E3), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on. |
| Purification Method | Protein G purified |
|---|---|
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
SIL1 Mouse mAb (Ab127610) - Western Blot
All lanes: SIL1 Mouse mAb (Ab127610) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 48, 54 kDa
The expression profile and molecular weight observed, which is due to glycosylation.
Exposure time: 57.2 s
SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)
SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)
SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using SIL1 Mouse mAb (Ab127610). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab127610, Dilution 1/50)
SIL1 Mouse mAb (Ab127610) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using SIL1 Mouse mAb (Ab127610). (Heatinduced epitope retrieval by 10 mM citric buffer, pH 6.0, 100 °C for 10 min, Ab127610, Dilution 1/50)
SIL1 Mouse mAb (Ab127610) - IF/ICC
SIL1 Mouse mAb (Ab127610) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIL1.
SIL1 Mouse mAb (Ab127610) - IF/ICC
Immunofluorescent staining of HT29 cells using SIL1 Mouse mAb (Ab127610).
SIL1 Mouse mAb (Ab127610) - Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY SIL1 (Red) or empty vector control plasmid (Blue) were immunostained with SIL1 Mouse mAb (Ab127610), and then analyzed by flow cytometry.
| Application | Dilution info |
|---|---|
| IHC | 1/100-1/500 |
| IF/ICC | 1/100 |
| WB | 1/200-1/1000 |
| Flow Cytometry | 1mg/ml |