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Lamin A/C Mouse mAb - Primary antibody, specific to Lamin A

    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Rat
  • Isotype: Mouse IgG2b
  • Clone Number: AB07/4G6
  • Conjugation: Unconjugated
In stock
Item Number
Ab210151
Grouped product items
SKU Size
Availability
Price Qty
Ab210151-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
Ab210151-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$109.90
Ab210151-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$169.90
Ab210151-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,099.90

mAb (AB07/4G6); Mouse anti Human Lamin A/C; WB, Flow cytometry, IF/ICC, ELISA; Unconjugated

Basic Description

Product Name Lamin A/C Mouse mAb - Primary antibody, specific to Lamin A
Synonyms 70 kDa lamin antibody | Cardiomyopathy dilated 1A (autosomal dominant) antibody | CDCD1 antibody | CDDC antibody | CMD1A antibody | CMT2B1 antibody | EMD2 antibody | FPL antibody | FPLD antibody | FPLD2 antibody | HGPS antibody | IDC antibody | Lamin A an
Specifications & Purity Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal
Host species Mouse
Specificity Lamin A
Immunogen Recombinant protein corresponding to Human Lamin A
Positive Control WB: HeLa, A549, MCF7, THP-1, K562 and C6 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells
Conjugation Unconjugated
Grade Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human Lamin A/C Antibody, clone AB07/4G6, could be used for WB, Flow cytometry, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

LMNA Tbio Prelamin-A/C (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Lamin A/C Mouse mAb (Ab210151) - Western Blot
All lanes: Lamin A/C Mouse Antibody (Ab210151) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 74, 65 kDa
Observed band size: 72, 65 kDa
Exposure time: 10.0 s

Lamin A/C Mouse mAb (Ab210151) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Lamin A/C Mouse mAb (Ab210151) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

Lamin A/C Mouse mAb (Ab210151) - ICC/IF
IF analysis of Lamin A/C (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Lamin A/C Mouse mAb (Ab210151) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/200

WB

1/20000 – 1/200000

Flow Cytometry

1/800 – 1/50000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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