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SKU | Size | Availability |
Price | Qty |
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Ab210151-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$39.90
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Ab210151-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$109.90
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Ab210151-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$169.90
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Ab210151-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,099.90
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mAb (AB07/4G6); Mouse anti Human Lamin A/C; WB, Flow cytometry, IF/ICC, ELISA; Unconjugated
Product Name | Lamin A/C Mouse mAb - Primary antibody, specific to Lamin A |
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Synonyms | 70 kDa lamin antibody | Cardiomyopathy dilated 1A (autosomal dominant) antibody | CDCD1 antibody | CDDC antibody | CMD1A antibody | CMT2B1 antibody | EMD2 antibody | FPL antibody | FPLD antibody | FPLD2 antibody | HGPS antibody | IDC antibody | Lamin A an |
Specifications & Purity | Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
Biochemical and Physiological Mechanisms | Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal |
Host species | Mouse |
Specificity | Lamin A |
Immunogen | Recombinant protein corresponding to Human Lamin A |
Positive Control | WB: HeLa, A549, MCF7, THP-1, K562 and C6 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells |
Conjugation | Unconjugated |
Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
Product Description |
Mouse anti Human Lamin A/C Antibody, clone AB07/4G6, could be used for WB, Flow cytometry, IF/ICC, ELISA and so on. |
Light Chain Type | kappa |
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SDS-PAGE | 150.0 KDa |
Purification Method | Protein A purified |
Concentration | 1.0 mg/mL |
Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
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Lamin A/C Mouse mAb (Ab210151) - Western Blot
All lanes: Lamin A/C Mouse Antibody (Ab210151) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 74, 65 kDa
Observed band size: 72, 65 kDa
Exposure time: 10.0 s
Lamin A/C Mouse mAb (Ab210151) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Lamin A/C Mouse mAb (Ab210151) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).
Lamin A/C Mouse mAb (Ab210151) - ICC/IF
IF analysis of Lamin A/C (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Lamin A/C Mouse mAb (Ab210151) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Application | Dilution info |
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IF/ICC | 1/200 |
WB | 1/20000 – 1/200000 |
Flow Cytometry | 1/800 – 1/50000 |
Starting at $49.90
Starting at $69.90
Starting at $39.90