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Grp75 Mouse mAb - Primary antibody, specific to Grp75, IgG2b

    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG2b
  • Clone Number: CD02/2E10
  • Conjugation: Unconjugated
In stock
Item Number
Ab181865
Grouped product items
SKU Size
Availability
Price Qty
Ab181865-10μl
10μl
≥10
$99.90
Ab181865-50μl
50μl
≥10
$189.90
Ab181865-100μl
100μl
9
$299.90
Ab181865-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (CD02/2E10); Mouse anti Human Grp75 Antibody; WB, ELISA, Flow, ICC/IF; Unconjugated

Basic Description

Product Name Grp75 Mouse mAb - Primary antibody, specific to Grp75, IgG2b
Synonyms 75 kDa glucose regulated protein antibody | 75 kDa glucose-regulated protein antibody | CSA antibody | Glucose Regulated Protein antibody | Grp 75 antibody | GRP-75 antibody | GRP75 antibody | GRP75_HUMAN antibody | Heat shock 70 kDa protein 9 antibody |
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
Host species Mouse
Specificity Grp75
Immunogen Recombinant protein corresponding to Human Grp75
Positive Control WB: HeLa, HEK293, Jurkat, MCF-7, HepG2, A431, A549, K562, Mouse Brain, NIH/3T3 and C6 Whole cell Lysates Flow: HeLa cells ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application

WB: 1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa

ICC/IF: 1/200

Flow: 1/200 - 1/25,000

Product Properties

Isotype IgG2b
Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

HSPA9 Tchem Stress-70 protein, mitochondrial (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 16.0 s 

Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 71 kDa
Exposure time: 2.3 s 

Grp75 Mouse mAb (Ab181865) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Grp75 Mouse mAb (Ab181865) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

Grp75 Mouse mAb (Ab181865) - ICC/IF
IF analysis of Grp75 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Grp75 Mouse mAb (Ab181865) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/200

WB

1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa

Flow

1/200 - 1/25,000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0404642 Certificate of Analysis Apr 23, 2024 Ab181865
ZJ24F0404641 Certificate of Analysis Apr 23, 2024 Ab181865
ZJ24F0404640 Certificate of Analysis Apr 23, 2024 Ab181865

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