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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab181865-10μl
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10μl |
≥10
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$99.90
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Ab181865-50μl
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50μl |
≥10
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$189.90
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Ab181865-100μl
|
100μl |
9
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$299.90
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Ab181865-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$1,999.90
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mAb (CD02/2E10); Mouse anti Human Grp75 Antibody; WB, ELISA, Flow, ICC/IF; Unconjugated
| Product Name | Grp75 Mouse mAb - Primary antibody, specific to Grp75, IgG2b |
|---|---|
| Synonyms | 75 kDa glucose regulated protein antibody | 75 kDa glucose-regulated protein antibody | CSA antibody | Glucose Regulated Protein antibody | Grp 75 antibody | GRP-75 antibody | GRP75 antibody | GRP75_HUMAN antibody | Heat shock 70 kDa protein 9 antibody | |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | Grp75 |
| Immunogen | Recombinant protein corresponding to Human Grp75 |
| Positive Control | WB: HeLa, HEK293, Jurkat, MCF-7, HepG2, A431, A549, K562, Mouse Brain, NIH/3T3 and C6 Whole cell Lysates Flow: HeLa cells ICC/IF: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Application WB: 1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa ICC/IF: 1/200 Flow: 1/200 - 1/25,000 |
| Isotype | IgG2b |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa
Exposure time: 16.0 s
Grp75 Mouse mAb (Ab181865) - Western Blot
All lanes: Grp75 Mouse Antibody (Ab181865) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 71 kDa
Exposure time: 2.3 s
Grp75 Mouse mAb (Ab181865) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Grp75 Mouse mAb (Ab181865) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).
Grp75 Mouse mAb (Ab181865) - ICC/IF
IF analysis of Grp75 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Grp75 Mouse mAb (Ab181865) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC |
1/200
|
| WB |
1/1,000 - 1/200,000; Predicted molecular weight: 73.7 KDa
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| Flow |
1/200 - 1/25,000
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Apr 23, 2024 | Ab181865 | |
| Certificate of Analysis | Apr 23, 2024 | Ab181865 | |
| Certificate of Analysis | Apr 23, 2024 | Ab181865 |
Starting at $66.90
Starting at $99.90