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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab213111-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$23.90
|
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Ab213111-25μg
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25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$46.90
|
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Ab213111-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$112.90
|
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Recombinant (P49A24); Rat anti Mouse CD3 Antibody (AF647); Flow Cytometry
| Product Name | Recombinant CD3 Antibody (AF647) - Primary antibody, specific to CD3 |
|---|---|
| Synonyms | CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b |
| Host species | Rat |
| Specificity | CD3 |
| Immunogen | γδTCR-positive T-T hybridoma D1 |
| Positive Control | Flow Cytometry: C57BL/6 mouse splenocytes |
| Conjugation | AF647 |
| Excitation(nm) | Red Laser(633nm) |
| Emission(nm) | 668nm |
| Ex/Em(nm) | Ex:650nm;Em:668nm |
| Grade | ExactAb™, Validated |
| Product Description |
Recombinant CD3 Antibody, clone P49A24, could be used for Flow Cytometry. |
| Light Chain Type | kappa |
|---|---|
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant CD3 Antibody (AF647) (Ab213111) - Flow Cytometry
Two-color flow cytometric analysis of CD3 expression on C57BL/6 Mouse splenocytes. C57BL/6 Mouse splenocytes cells were stained with CD45R Rat mAb (FITC) (Ab191667) and either Rat IgG (AF647) (Ab190071; Left panel) or Recombinant CD3 Antibody (AF647) (Ab213111; Right panel) at 0.3μg/test.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. |
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