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CD3 Rat mAb (Biotin) - Primary antibody, specific to CD3, Rat IgG2b

COA COA
    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2b
  • Clone Number: 17A2
  • Conjugation: Biotin
In stock
Item Number
Ab185405
Grouped product items
SKU Size
Availability
 Price Qty
Ab185405-10μg
10μg
2
$11.90
Ab185405-25μg
25μg
2
$22.90
Ab185405-100μg
100μg
1
$69.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (17A2); Rat anti Mouse CD3 Antibody (Biotin); Flow

View related series
Click to view target information: CD3G
Accession#:P11942 (8) CD3G (7) Ctg-3 (6) Ctg3 (6) Flow Cytometry (1158) Gene ID:12502 (8) T3g (6)

Basic Description

Product Name CD3 Rat mAb (Biotin) - Primary antibody, specific to CD3, Rat IgG2b
Synonyms CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Host species Rat
Specificity CD3
Immunogen γδTCR-positive T-T hybridoma D1
Positive Control Flow: Mouse splenocytes.
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Flow: ≤0.3 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Product Properties

Isotype Rat IgG2b
Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD3G Tclin T-cell surface glycoprotein CD3 gamma chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD3 Rat mAb (Biotin) (Ab185405) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Rat mAb (Biotin) (Ab185405) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).

CD3 Rat mAb (Biotin) (Ab185405) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD3 Rat mAb (Biotin) (Ab185405) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0809130 Certificate of Analysis Aug 02, 2024 Ab185405
ZJ24F0809129 Certificate of Analysis Aug 02, 2024 Ab185405
ZJ24F0809131 Certificate of Analysis Aug 02, 2024 Ab185405
ZJ24R0700849 Certificate of Analysis Jul 26, 2024 Ab185405

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