Grouped product items

SKU

Size

Availability

Price

Qty

Ab213110-10μg

10μg

Available within 8-12 weeks(?)

$49.90

Ab213110-25μg

25μg

Available within 8-12 weeks(?)

$89.90

Ab213110-100μg

100μg

Available within 8-12 weeks(?)

$203.90

Basic Description

Product Name	Recombinant CD3 Antibody (AF405) - Primary antibody, specific to CD3
Synonyms	CD_antigen: CD3e \| CD3 antigen, delta subunit \| CD3 \| CD3d antigen \| CD3d antigen, delta polypeptide (TiT3 complex) \| CD3d molecule, delta (CD3-TCR complex) \| CD3-DELTA \| CD3e antigen \| CD3e antigen, epsilon polypeptide (TiT3 complex) \| CD3e molecule, eps
Specifications & Purity	ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms	Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b
Host species	Rat
Specificity	CD3
Immunogen	γδTCR-positive T-T hybridoma D1
Positive Control	Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation	AF405
Excitation(nm)	Violet Laser(405nm)
Emission(nm)	422nm
Ex/Em(nm)	Ex:401nm;Em:422nm
Grade	ExactAb™, Validated
Product Description	Recombinant CD3 Antibody, clone P49A24, could be used for Flow Cytometry.

Product Properties

Light Chain Type	kappa
Purification Method	Protein G purified
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD3G Tclin T-cell surface glycoprotein CD3 gamma chain (0 Activities)

Discover Target: CD3G

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: Recombinant CD3 Antibody (AF405) (Ab213110)

Recombinant CD3 Antibody (AF405) (Ab213110) - Flow Cytometry
Two-color flow cytometric analysis of CD3 expression on C57BL/6 Mouse splenocytes. C57BL/6 Mouse splenocytes cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (AF405) (Ab190070; Left panel) or Recombinant CD3 Antibody (AF405) (Ab213110; Right panel) at 0.1μg/test.

Flow Cytometry: Recombinant CD3 Antibody (AF405) (Ab213110)

Recombinant CD3 Antibody (AF405) (Ab213110) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD3 Antibody (AF405) (Ab213110) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Recombinant CD3 Antibody (AF405) (Ab213110)

Recombinant CD3 Antibody (AF405) (Ab213110) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD3 Antibody (AF405) (Ab213110) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).

Application

Application	Dilution info
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Alternative Products

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Solution Calculators

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Recombinant CD3 Antibody (AF405) - Primary antibody, specific to CD3

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

SKU	Size	Availability	Price
Ab213110-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$49.90
Ab213110-25μg	25μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$89.90
Ab213110-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$203.90

Recombinant CD3 Antibody (AF405) - Primary antibody, specific to CD3

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews