Grouped product items

SKU

Size

Availability

Price

Qty

Ab213312-10μg

10μg

Available within 8-12 weeks(?)

$19.90

Ab213312-25μg

25μg

Available within 8-12 weeks(?)

$39.90

Ab213312-100μg

100μg

Available within 8-12 weeks(?)

$119.90

Basic Description

Product Name	Recombinant CD16/CD32 Antibody (Biotin) - Primary antibody, specific to CD16/32
Synonyms	CD32 \| Fcgr2 \| Fcr-2 \| Fcr-3 \| Ly-17 \| LyM-1 \| fcRII \| FcgRII \| Fcgr2a \| Ly-m20 \| Fc[g]RII \| F630109E10Rik \| CD16 \| CD16A \| CD16-II \| FCG3 \| FCGR3 \| FCGR3A \| FCGRIII \| FcGRIIIA \| FCR-10 \| FCRIII \| FCRIIIA \| IGFR3 \| IMD20 \| Low affinity immunoglobulin gamm
Specifications & Purity	ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms	CD16 and CD32 serve as low affinity receptors for IgG Fc constant regions and are involved in regulating various cellular functions including antibody-dependent cellular toxicity (ADCC), phagocytosis, effector cell degranulation, and B cell proliferation.
Host species	Rat
Specificity	CD16/32
Positive Control	Flow: C57BL/6 mouse splenocytes
Conjugation	Biotin
Grade	ExactAb™, Validated
Product Description	Recombinant CD16/CD32 Antibody, clone P05C29, could be used for Flow Cytometry and so on.

Product Properties

Light Chain Type	kappa
Purification Method	Protein A purified
Concentration	0.5 mg/mL
Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

FCGR2B Tbio Low affinity immunoglobulin gamma Fc region receptor II-b (0 Activities)

Discover Target: FCGR2B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Flow Cytometry: Recombinant CD16/CD32 Antibody (Biotin) (Ab213312)

Recombinant CD16/CD32 Antibody (Biotin) (Ab213312) - Flow Cytometry
Two-color flow cytometric analysis of CD16/CD32 expression on C57BL/6 Mouse splenocytes. C57BL/6 Mouse splenocytes cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (Biotin) (Ab180310; Left panel) or Recombinant CD16/CD32 Antibody (Biotin) (Ab213312; Right panel) at 0.2μg/test. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark.

Application

Application	Dilution info
Flow Cytometry	≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Alternative Products

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Solution Calculators

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Recombinant CD16/CD32 Antibody (Biotin) - Primary antibody, specific to CD16/32

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

SKU	Size	Availability	Price
Ab213312-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$19.90
Ab213312-25μg	25μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$39.90
Ab213312-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$119.90

Recombinant CD16/CD32 Antibody (Biotin) - Primary antibody, specific to CD16/32

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews