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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab210978-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$12.90
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Ab210978-50μg
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50μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$38.90
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Ab210978-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$61.90
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Ab210978-1mg
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1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
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Recombinant (P05C29); Rat anti Mouse CD16/CD32 Antibody; Flow Cytometry, Blocking, ELISA; Unconjugated
| Product Name | Recombinant CD16/CD32 Antibody - Primary antibody, specific to CD16/32 |
|---|---|
| Synonyms | CD32 | Fcgr2 | Fcr-2 | Fcr-3 | Ly-17 | LyM-1 | fcRII | FcgRII | Fcgr2a | Ly-m20 | Fc[g]RII | F630109E10Rik | CD16 | CD16A | CD16-II | FCG3 | FCGR3 | FCGR3A | FCGRIII | FcGRIIIA | FCR-10 | FCRIII | FCRIIIA | IGFR3 | IMD20 | Low affinity immunoglobulin gamm |
| Specifications & Purity | Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA |
| Biochemical and Physiological Mechanisms | CD16 and CD32 serve as low affinity receptors for IgG Fc constant regions and are involved in regulating various cellular functions including antibody-dependent cellular toxicity (ADCC), phagocytosis, effector cell degranulation, and B cell proliferation. |
| Host species | Rat |
| Specificity | CD16/32 |
| Positive Control | Flow Cytometry: C57BL/6 splenocytes |
| Conjugation | Unconjugated |
| Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated |
| Product Description |
Recombinant CD16/CD32 Antibody, clone P05C29, could be used for Flow Cytometry, Blocking, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Recombinant CD16/CD32 Antibody (Ab210978) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD16/CD32 with Recombinant CD16/CD32 Antibody (Ab210978) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).
Recombinant CD16/CD32 Antibody (Ab210978) - Flow Cytometry
Flow cytometry analysis of the expression of CD16/CD32 on mouse spleen cells and demonstration of FCγR-mediated non-specific staining.
Left: Flow cytometry analysis of CD16/CD32 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with recombinant CD16/CD32 antibody (Ab210978) at 0.2 μg/mL for 1 hour at 4°C. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
Right: C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) blocked with purified recombinant CD16/CD32 antibody (red) or 5% mouse serum (green). Blue - No blocking control, cells were stained with rat IgG (PE) without any blocking reagent; Black - Unlabelled control, cells without incubation with any antibody.
Recombinant CD16/CD32 Antibody (Ab210978) - SEC
The purity of Recombinant CD16/CD32 Antibody (Ab210978) is more than 95% verified by HPLC.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.5 µg per million cells in 100 µl volume |
| Blocking | 0.1 µg per million cells in 100 µL volume |
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