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Recombinant CD16/CD32 Antibody - Primary antibody, specific to Mouse CD16/CD32

    Application:
  • Blocking
  • ELISA
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2b
  • Clone Number: P95D31
  • Conjugation: Unconjugated
In stock
Item Number
Ab212150
Grouped product items
SKU Size
Availability
Price Qty
Ab212150-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$19.90
Ab212150-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$62.90
Ab212150-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90

Recombinant (P95D31); Rat anti Mouse CD16/CD32 Antibody; Flow Cytometry, ELISA, Blocking; Unconjugated

Basic Description

Product Name Recombinant CD16/CD32 Antibody - Primary antibody, specific to Mouse CD16/CD32
Synonyms CD32 | Fcgr2 | Fcr-2 | Fcr-3 | Ly-17 | LyM-1 | fcRII | FcgRII | Fcgr2a | Ly-m20 | Fc[g]RII | F630109E10Rik | CD16 | CD16A | CD16-II | FCG3 | FCGR3 | FCGR3A | FCGRIII | FcGRIIIA | FCR-10 | FCRIII | FCRIIIA | IGFR3 | IMD20 | Low affinity immunoglobulin gamm
Specifications & Purity Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms CD16: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b. Mediates neutrophil activation by IgG complexes redundantly with Fcgr4. CD32: Receptor for the Fc region of complexed immunogl
Host species Rat
Specificity Mouse CD16/CD32
Immunogen Mouse BALB/c Macrophage J774
Positive Control Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation Unconjugated
Grade Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated
Note 此产品可替代 A412018
Product Description

Recombinant CD16/CD32 Antibody, clone P95D31, could be used for Flow Cytometry, ELISA, Blocking and so on.

Product Properties

Light Chain Type lambda
SDS-PAGE 150 kDa
Purification Method Protein A purified
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

FCGR2A Tbio Low affinity immunoglobulin gamma Fc region receptor II-a (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
FCGR3A Tclin Low affinity immunoglobulin gamma Fc region receptor III-A (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant CD16/CD32 Antibody (Ab212150) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD16/CD32 with Recombinant CD16/CD32 Antibody (Ab212150) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Recombinant CD16/CD32 Antibody (Ab212150) - Flow Cytometry
Flow cytometry analysis of the expression of CD16/CD32 on mouse spleen cells and demonstration of FCγR-mediated non-specific staining.
Left: Flow cytometry analysis of CD16/CD32 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD16/CD32 Antibody (Ab212150) at 0.2 μg/mL for 1 hour at 4°C. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
Right: C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) blocked with purified Recombinant CD16/CD32 Antibody (red) or 5% mouse serum (green). Blue - No blocking control, cells were stained with rat IgG (PE) without any blocking reagent; Black - Unlabelled control, cells without incubation with any antibody.

Recombinant CD16/CD32 Antibody (Ab212150) - SEC
The purity of Recombinant CD16/CD32 Antibody (Ab212150) is more than 95% verified by HPLC.

Application

Application Dilution info
Flow Cytometry

≤0.25 µg per  10⁶ cells in 100 µl volume

Blocking

0.25 µg per million cells in 100 µL volume

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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