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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab124756-10μl
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10μl |
1
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$79.90
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Ab124756-50μl
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50μl |
1
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$219.90
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Ab124756-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
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Ab124756-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,099.90
|
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mAb (K39S7K); Mouse anti Human RBCK1 Antibody; WB; Unconjugated
| Product Name | RBCK1 Mouse mAb - Primary antibody, specific to RBCK1, Mouse IgG1 |
|---|---|
| Synonyms | Chromosome 20 open reading frame 18 antibody | HBV associated factor 4antibody | HBV-associated factor 4 antibody | Heme-oxidized IRP2 ubiquitin ligase 1 antibody | Hepatitis B virus X associated protein 4 antibody | Hepatitis B virus X-associated protein |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both h |
| Host species | Mouse |
| Specificity | RBCK1 |
| Immunogen | Recombinant fusion protein of human RBCK1. |
| Positive Control | WB: Jurkat, HEK293, A375 and A549 cell lysates. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human RBCK1 Antibody, Monoclonal (K39S7K), could be used for WB and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
RBCK1 Mouse mAb (Ab124756) - Western Blot
All lanes: RBCK1 Mouse mAb (Ab124756) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
The expression molecular weight observed is consistent with what has been described in the literature (PMID: 31209050, PMID: 27006117).
The higher MW (57 kDa) band is the ubiquitylated form of RBCK1 (PMID: 31209050).
Bands below 57 kDa are cleaved RBCK1 (PMID: 27006117).
Exposure time: 65.7 s
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |