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RBCK1 Mouse mAb - Primary antibody, specific to RBCK1, Mouse IgG1

    Application:
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: K39S7K
  • Conjugation: Unconjugated
In stock
Item Number
Ab124756
Grouped product items
SKU Size
Availability
Price Qty
Ab124756-10μl
10μl
1
$79.90
Ab124756-50μl
50μl
1
$219.90
Ab124756-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab124756-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,099.90

mAb (K39S7K); Mouse anti Human RBCK1 Antibody; WB; Unconjugated

Basic Description

Product Name RBCK1 Mouse mAb - Primary antibody, specific to RBCK1, Mouse IgG1
Synonyms Chromosome 20 open reading frame 18 antibody | HBV associated factor 4antibody | HBV-associated factor 4 antibody | Heme-oxidized IRP2 ubiquitin ligase 1 antibody | Hepatitis B virus X associated protein 4 antibody | Hepatitis B virus X-associated protein
Specifications & Purity ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Biochemical and Physiological Mechanisms Acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both h
Host species Mouse
Specificity RBCK1
Immunogen Recombinant fusion protein of human RBCK1.
Positive Control WB: Jurkat, HEK293, A375 and A549 cell lysates.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human RBCK1 Antibody, Monoclonal (K39S7K), could be used for WB and so on.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

RBCK1 Tbio RanBP-type and C3HC4-type zinc finger-containing protein 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

RBCK1 Mouse mAb (Ab124756) - Western Blot
All lanes: RBCK1 Mouse mAb (Ab124756) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 57 kDa
The expression molecular weight observed is consistent with what has been described in the literature (PMID: 31209050, PMID: 27006117).
The higher MW (57 kDa) band is the ubiquitylated form of RBCK1 (PMID: 31209050).
Bands below 57 kDa are cleaved RBCK1 (PMID: 27006117).
Exposure time: 65.7 s

Application

Application Dilution info
WB

1/500-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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