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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab124145-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$89.90
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Ab124145-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$269.90
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Ab124145-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$429.90
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Ab124145-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$3,199.90
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mAb (1694CT210.218.54); Mouse anti Human Rab20 Antibody; WB, Flow Cytometry; Unconjugated
| Product Name | Rab20 Mouse mAb - Primary antibody, specific to Rab20, Mouse IgG2a |
|---|---|
| Synonyms | FLJ20429 antibody | RAB20 antibody | RAB20 member RAS oncogene family antibody | RAB20_HUMAN antibody | Ras related protein Rab20 antibody | Ras-related protein Rab-20 antibody |
| Specifications & Purity | Carrier Free, ExactAb™, High Performance, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. |
| Host species | Mouse |
| Specificity | Rab20 |
| Immunogen | Recombinant protein of human RAB20 (AA 1-190) |
| Positive Control | WB: THP-1, K562 and MCF7 cell lysates. Flow: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human Rab20 Antibody, Monoclonal (1694CT210.218.54), could be used for WB, Flow Cytometry and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Rab20 Mouse mAb (Ab124145) - Western Blot
All lanes: Rab20 Mouse mAb (Ab124145) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti Mouse HRP (Ab179001) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 29 kDa
Exposure time: 60.0 s
Rab20 Mouse mAb (Ab124145) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Rab20 Mouse mAb (Ab124145) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Rab20 Mouse mAb (Ab124145) (1/25 dilution) for 60 min at 37 ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37 ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1/25 |
| WB | 1/500-1/4000 |