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Rab20 Mouse mAb - Primary antibody, specific to Rab20, Mouse IgG2a

    Application:
  • Flow Cytometry
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse
  • Isotype: Mouse IgG2a
  • Clone Number: 1694CT210.218.54
  • Conjugation: Unconjugated
In stock
Item Number
Ab124145
Grouped product items
SKU Size
Availability
Price Qty
Ab124145-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab124145-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$269.90
Ab124145-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$429.90
Ab124145-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,199.90

mAb (1694CT210.218.54); Mouse anti Human Rab20 Antibody; WB, Flow Cytometry; Unconjugated

Basic Description

Product Name Rab20 Mouse mAb - Primary antibody, specific to Rab20, Mouse IgG2a
Synonyms FLJ20429 antibody | RAB20 antibody | RAB20 member RAS oncogene family antibody | RAB20_HUMAN antibody | Ras related protein Rab20 antibody | Ras-related protein Rab-20 antibody
Specifications & Purity Carrier Free, ExactAb™, High Performance, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
Host species Mouse
Specificity Rab20
Immunogen Recombinant protein of human RAB20 (AA 1-190)
Positive Control WB: THP-1, K562 and MCF7 cell lysates. Flow: HeLa cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human Rab20 Antibody, Monoclonal (1694CT210.218.54), could be used for WB, Flow Cytometry and so on.

Product Properties

Isotype Mouse IgG2a
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

RAB20 Tbio Ras-related protein Rab-20 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Rab20 Mouse mAb (Ab124145) - Western Blot
All lanes: Rab20 Mouse mAb (Ab124145) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti Mouse HRP (Ab179001) at 1/10000 dilution

Predicted band size: 26 kDa
Observed band size: 29 kDa
Exposure time: 60.0 s

Rab20 Mouse mAb (Ab124145) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Rab20 Mouse mAb (Ab124145) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Rab20 Mouse mAb (Ab124145) (1/25 dilution) for 60 min at 37 ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37 ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Application

Application Dilution info
Flow Cytometry

1/25

WB

1/500-1/4000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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