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PPP1A/PPP1CA Mouse mAb - Primary antibody, specific to PPP1A/PPP1CA, Mouse IgG1

    Application:
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 1H1-F7-D8
  • Conjugation: Unconjugated
In stock
Item Number
Ab122816
Grouped product items
SKU Size
Availability
Price Qty
Ab122816-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab122816-50μl
50μl
1
$179.90
Ab122816-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab122816-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

mAb (1H1-F7-D8); Mouse anti Human PPP1A/PPP1CA Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product Name PPP1A/PPP1CA Mouse mAb - Primary antibody, specific to PPP1A/PPP1CA, Mouse IgG1
Synonyms Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody | Catalytic subunit antibody | EC 3.1.3.16 antibody | MGC15877 antibody | MGC1674 antibody | PP 1A antibody | PP-1A antibody | PP1A antibody | PP1A_HUMAN antibody | P
Specifications & Purity ExactAb™, Validated, 1.0 mg/mL
Host species Mouse
Specificity PPP1A/PPP1CA
Immunogen Purified recombinant human PPP1A protein fragments expressed in E.coli (AA 1-330).
Positive Control WB: Hep G2, MCF7, HeLa, THP-1, A549, Raji, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Mouse anti Human PPP1A/PPP1CA Antibody, Monoclonal (1H1-F7-D8), could be used for WB, ICC/IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PPP1CA Tchem Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
PPP1CA Tchem Serine/threonine protein phosphatase PP1-alpha catalytic subunit (133 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

PPP1A/PPP1CA Mouse mAb (Ab122816) - Western Blot
All lanes: PPP1A/PPP1CA Mouse mAb (Ab122816) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 35, 40 kDa
Exposure time: 2.0 s

PPP1A/PPP1CA Mouse mAb (Ab122816) - IF/ICC
Immunocytochemistry analysis of PP1C alpha in HeLa cells using PPP1A/PPP1CA Mouse mAb (Ab122816).

Application

Application Dilution info
IF/ICC

1/50-1/200

WB

1/500-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1202124 Certificate of Analysis Dec 20, 2023 Ab122816
ZJ23F1202123 Certificate of Analysis Dec 20, 2023 Ab122816

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