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PD1 Mouse mAb (Biotin) - Primary antibody, specific to PD1, Mouse IgG1

COA COA
    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: EH12.2H7
  • Conjugation: Biotin
In stock
Item Number
Ab179528
Grouped product items
SKU Size
Availability
 Price Qty
Ab179528-10μg
10μg
10
$69.90
Ab179528-25μg
25μg
6
$119.90
Ab179528-100μg
100μg
1
$299.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (EH12.2H7); Mouse anti Human PD1 Antibody (Biotin); Flow

View related series
Click to view target information: PDCD1
Accession#:Q15116 (38) CD279 (28) Flow Cytometry (1158) Gene ID:5133 (38) hPD-1 (4) hPD-l (4) hSLE1 (4) PD-1 (9) PD1 (37) PDCD1 (39) SLEB2 (4)

Basic Description

Product Name PD1 Mouse mAb (Biotin) - Primary antibody, specific to PD1, Mouse IgG1
Synonyms CD279 | hPD-1 | PD1 | PD-1 | PD1hPD-l | PDCD1 | programmed cell death 1 | programmed cell death protein 1 | Protein PD-1 | SLEB2 | CD279 antibody | CD279 antigen antibody | hPD 1 antibody | CD279 antigen | hPD l antibody | hPD-1 antibody | hSLE1 antibody
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Host species Mouse
Specificity PD1
Immunogen Recombinant protein of human CD279
Positive Control Flow: PMA and lonomycin stimulated (overnight) MOLT4
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Application:
Flow: ≤0.5 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

PDCD1 Tclin Programmed cell death protein 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
PDCD1 Tclin Programmed cell death protein 1 (14 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

PD1 Mouse mAb (Biotin) (Ab179528) - Flow Cytometry
MOLT-4 cells treated with 500 ng/mL Ionomycin and 10 ng/mL Phorbol-12-myristate-13-acetate (PMA) for 24 hours (right panel) are stained with PD1 Mouse mAb (Biotin) (Ab179528) (red) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody. Untreated MOLT-4 cells were used as control (left panel).

Application

Application Dilution info
Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0606970 Certificate of Analysis Jun 18, 2024 Ab179528
ZJ24F0606969 Certificate of Analysis Jun 18, 2024 Ab179528
ZJ24F0606968 Certificate of Analysis Jun 18, 2024 Ab179528
ZJ24R0600663 Certificate of Analysis Jun 09, 2024 Ab179528

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