Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab117026-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$79.90
|
|
|
Ab117026-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$219.90
|
|
|
Ab117026-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
|
|
|
Ab117026-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
|
mAb (K1E005_8C10); Mouse anti Human NAGK Antibody; WB, IHC, IP; Unconjugated
| Product Name | NAGK Mouse mAb - Primary antibody, specific to NAGK, Mouse IgG1 |
|---|---|
| Synonyms | EC 2.7.1.59 antibody | GlcNAc kinase antibody | GNK antibody | HSA242910 antibody | N acetylglucosamine kinase antibody | N-acetyl-D-glucosamine kinase antibody | N-acetylglucosamine kinase antibody | nagK antibody | NAGK_HUMAN antibody | EC 2.7.1.59 | Gl |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | NAGK |
| Immunogen | Recombinant protein of human NAGK (AA 1-344). |
| Positive Control | WB: HeLa, Jurkat, HEK293, A431 and C6 cell lysates. IHC: Human tonsil tissue. IP: HeLa lysates. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human NAGK Antibody, Monoclonal (K1E005_8C10), could be used for WB, IHC, IP and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
NAGK Mouse mAb (Ab117026) - Western Blot
All lanes: NAGK Mouse mAb (Ab117026) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 11.4 s
NAGK Mouse mAb (Ab117026) - Western Blot
All lanes: NAGK Mouse mAb (Ab117026) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 80.6 s
NAGK Mouse mAb (Ab117026) - IHC
IHC analysis of tonsil tissue by NAGK Mouse mAb (Ab117026).IHC was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NAGK Mouse mAb (Ab117026) at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Germinal center cells and non-germinal center cells are positively stained at the cytoplasm.
NAGK Mouse mAb (Ab117026) - IP
Immunoprecipitation was performed by incubation of 2.5 µg NAGK Mouse mAb (Ab117026) with HeLa lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. NAGK Mouse mAb (Ab117026) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody.Lane 1: HeLa lysateLane 2: NAGK immunoprecipitated from HeLa lysate by NAGK Mouse mAb (Ab117026)
Lane 3: The isotype control antibody instead of NAGK Mouse mAb (Ab117026) in HeLa lysate.Result: NAGK Mouse mAb (Ab117026) can immunoprecipitate NAGK.
| Application | Dilution info |
|---|---|
| WB |
1/1000
|
| IP | 1/100 |
| IHC | 1/50-1/250 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Aug 20, 2024 | Ab117026 | |
| Certificate of Analysis | Aug 20, 2024 | Ab117026 |