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NAGK Mouse mAb - Primary antibody, specific to NAGK, Mouse IgG1

    Application:
  • IHC
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Human,Rat
  • Isotype: Mouse IgG1
  • Clone Number: K1E005_8C10
  • Conjugation: Unconjugated
In stock
Item Number
Ab117026
Grouped product items
SKU Size
Availability
Price Qty
Ab117026-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab117026-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
Ab117026-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab117026-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

mAb (K1E005_8C10); Mouse anti Human NAGK Antibody; WB, IHC, IP; Unconjugated

Basic Description

Product Name NAGK Mouse mAb - Primary antibody, specific to NAGK, Mouse IgG1
Synonyms EC 2.7.1.59 antibody | GlcNAc kinase antibody | GNK antibody | HSA242910 antibody | N acetylglucosamine kinase antibody | N-acetyl-D-glucosamine kinase antibody | N-acetylglucosamine kinase antibody | nagK antibody | NAGK_HUMAN antibody | EC 2.7.1.59 | Gl
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species Mouse
Specificity NAGK
Immunogen Recombinant protein of human NAGK (AA 1-344).
Positive Control WB: HeLa, Jurkat, HEK293, A431 and C6 cell lysates. IHC: Human tonsil tissue. IP: HeLa lysates.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human NAGK Antibody, Monoclonal (K1E005_8C10), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/50-1/250
IP: 1/100
Protein Function
Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -80°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

NAGK Tbio N-acetyl-D-glucosamine kinase (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

NAGK Mouse mAb (Ab117026) - Western Blot
All lanes: NAGK Mouse mAb (Ab117026) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 11.4 s

NAGK Mouse mAb (Ab117026) - Western Blot
All lanes: NAGK Mouse mAb (Ab117026) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 80.6 s

NAGK Mouse mAb (Ab117026) - IHC
IHC analysis of tonsil tissue by NAGK Mouse mAb (Ab117026).IHC was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NAGK Mouse mAb (Ab117026) at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Germinal center cells and non-germinal center cells are positively stained at the cytoplasm.

NAGK Mouse mAb (Ab117026) - IP
Immunoprecipitation was performed by incubation of 2.5 µg NAGK Mouse mAb (Ab117026) with HeLa lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. NAGK Mouse mAb (Ab117026) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody.Lane 1: HeLa lysateLane 2: NAGK immunoprecipitated from HeLa lysate by NAGK Mouse mAb (Ab117026)
Lane 3: The isotype control antibody instead of NAGK Mouse mAb (Ab117026) in HeLa lysate.Result: NAGK Mouse mAb (Ab117026) can immunoprecipitate NAGK.

Application

Application Dilution info
WB

1/1000

IP

1/100

IHC

1/50-1/250

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0809840 Certificate of Analysis Aug 20, 2024 Ab117026
ZJ24F0809839 Certificate of Analysis Aug 20, 2024 Ab117026

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