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MKRN2 Mouse mAb - Primary antibody, specific to MKRN2, Mouse IgG1

    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: F56S631
  • Conjugation: Unconjugated
In stock
Item Number
Ab115527
Grouped product items
SKU Size
Availability
Price Qty
Ab115527-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab115527-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
Ab115527-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab115527-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,099.90

mAb (F56S631); Mouse anti Human MKRN2 Antibody; WB, IHC, IF, ICC, Flow; Unconjugated

Basic Description

Product Name MKRN2 Mouse mAb - Primary antibody, specific to MKRN2, Mouse IgG1
Synonyms HSPC070 antibody | Makorin 2 antibody | Makorin ring finger protein 2 antibody | Makorin2 antibody | MKRN 2 antibody | RING finger protein 62 antibody | RNF 62 antibody | RNF62 antibody | RNF62 | HSPC070 | Makorin 2 antibody | HSPC070 antibody | Makorin r
Specifications & Purity Carrier Free, ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Relevance: MKRN2 (Makorin 2) contains 4 C3H1 type zinc fingers.
Host species Mouse
Specificity MKRN2
Immunogen Recombinant protein of human Makorin-2
Positive Control WB: Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human heart tissue. IF/ICC: U-2 OS cells. Flow: K562 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human MKRN2 Antibody, Monoclonal (F56S631), could be used for WB, IHC, IF, ICC, Flow and so on.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MKRN2 Tbio Probable E3 ubiquitin-protein ligase makorin-2 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

MKRN2 Mouse mAb (Ab115527) - Western Blot
All lanes: MKRN2 Mouse mAb (Ab115527) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 60.0 s

MKRN2 Mouse mAb (Ab115527) - IHC
Immunohistochemical analysis of Makorin-2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with MKRN2 Mouse mAb (Ab115527) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

MKRN2 Mouse mAb (Ab115527) - IF/ICC
Immunofluorescent analysis of Makorin-2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with MKRN2 Mouse mAb (Ab115527) in 3% BSA-PBS and incubated overnight at 4℃ in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

MKRN2 Mouse mAb (Ab115527) - Flow
Flow cytometric analysis of K562 cells using MKRN2 Mouse mAb (Ab115527). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37℃ for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37℃ for 40 min. Isotype control antibody (blue line) was used under the same condition.

Application

Application Dilution info
Flow Cytometry

1/10-1/50

IHC

1/50-1/200

IF/ICC

1/10 - 1/50

WB

1/500-1/2000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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