Grouped product items

SKU

Size

Availability

Price

Qty

Ab109567-10μl

10μl

Available within 8-12 weeks(?)

$66.90

Ab109567-20μl

20μl

$99.90

Ab109567-50μl

50μl

$189.90

Ab109567-100μl

100μl

$299.90

Ab109567-1ml

1ml

Available within 8-12 weeks(?)

$2,199.90

Basic Description

Product Name	IGFBP3 Mouse mAb - Primary antibody, specific to IGFBP3, Mouse IgG1
Synonyms	Acid stable subunit of the 140 K IGF complex antibody \| Binding protein 29 antibody \| Binding protein 53 antibody \| BP 53 antibody \| BP53 antibody \| Growth hormone dependent binding proteinantibody \| IBP 3 antibody \| IBP3 antibody \| IGF binding protein 3
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species	Mouse
Specificity	IGFBP3
Immunogen	Recombinant IGFBP3 expressed in E.coli (AA 135-277)
Positive Control	WB: A431 and Hep G2 cell lysates.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human IGFBP3 Antibody, Monoclonal (D15), could be used for WB and so on. Application: WB: 0.5-2µg/mL Protein Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Insulin like growth factor binding protein 3 (IGFBP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGFBP3 is fused to the signal peptide of CD33. Human IGFBP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. Insulin like growth factor binding protein 3 (IGFBP3) can modulate the mitogenic and metabolic effects of the insulin like growth factors (IGFs). Insulin like growth factor binding protein 3 (IGFBP3) is expressed in multiple tissues. The highest expression level is found in the non paranchymal cells of the liver. The expression levels are higher during extrauterine life and peak during puberty. Insulin like growth factors (IGFs) and IGF binding proteins (IGFBPs) play important roles in cell growth and differentiation. IGFBP3 is one of the factors in serum that is responsible for high serum induced apoptosis in PC3 cells, a prostate cancer cell line. IGFBP3 is important in controlling glucose homeostasis with increased urinary levels in type I diabetes with persistent microalbuminuria.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A + Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

IGFBP3 Tchem Insulin-like growth factor-binding protein 3 (0 Activities)

Discover Target: IGFBP3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: IGFBP3 Mouse mAb (Ab109567)

IGFBP3 Mouse mAb (Ab109567) - Western Blot
All lanes: IGFBP3 Mouse mAb (Ab109567) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 37 kDa
Exposure time: 9.1 s

Application

Application	Dilution info
WB	0.5-2µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1201972	Certificate of Analysis	Dec 13, 2023	Ab109567
ZJ23F1201973	Certificate of Analysis	Dec 13, 2023	Ab109567
ZJ23F1201974	Certificate of Analysis	Dec 13, 2023	Ab109567

Documents & Articles

Immunohistochemistry staining with samples in paraffin (IHC-P)

Categories: Protocols

Tags:

Monoclonal antibody Paraffin Section Immunohistochemistry/IHC

Guidelines of Primary Antibodies for Western Blotting

Categories: Protocols

Tags:

Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls

Protocol of Western Blotting For High Molecular Weights

Categories: Protocols

Tags:

Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody

Solution Calculators

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IGFBP3 Mouse mAb - Primary antibody, specific to IGFBP3, Mouse IgG1

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

SKU	Size	Availability	Price
Ab109567-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$66.90
Ab109567-20μl	20μl	3	$99.90
Ab109567-50μl	50μl	4	$189.90
Ab109567-100μl	100μl	2	$299.90
Ab109567-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,199.90

IGFBP3 Mouse mAb - Primary antibody, specific to IGFBP3, Mouse IgG1

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews