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Hsp60 Mouse mAb - Primary antibody, specific to Hsp60, Mouse IgG1

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: EF02/2E10
  • Conjugation: Unconjugated
In stock
Item Number
Ab169190
Grouped product items
SKU Size
Availability
 Price Qty
Ab169190-10μl
10μl
≥10

$47.90

$59.90
Save $12.00 (20.03%)
Ab169190-50μl
50μl
10
$169.90
Ab169190-100μl
100μl
9
$269.90
Ab169190-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,599.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (EF02/2E10); Mouse anti Human Hsp60 Antibody; WB, ICC/IF, Flow, ELISA; Unconjugated

View related series
Click to view target information: HSPD1
Accession#:P10809 (7) Flow Cytometry (1158) Gene ID:3329 (7) Hsp60 (7) HSPD1 (7)

Basic Description

Product Name Hsp60 Mouse mAb - Primary antibody, specific to Hsp60, Mouse IgG1
Synonyms 60 kDa chaperonin antibody | 60 kDa heat shock protein, mitochondrial antibody | CH60_HUMAN antibody | Chaperonin 60 antibody | Chaperonin, 60-KD antibody | CPN60 antibody | fa04a05 antibody | GROEL antibody | heat shock 60kDa protein 1 (chaperonin) antib
Specifications & Purity Validated, High performance, Carrier Free, Azide Free, ≥95%(SDS-PAGE), 1.0 mg/mL
Host species Mouse
Specificity Hsp60
Immunogen Recombinant full length protein corresponding to Human Hsp60
Positive Control WB: HEK293, HeLa, HepG2, A549, MOTL-4, MCF-7, Jurkat, K562, A431 and THP-1 Whole cell Lysates ICC/IF: U-2 OS cells Flow: Jurkat cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, High Performance, Validated
Product Description

Application
WB: 1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa
ICC/IF: 1/200
Flow: 1/800 - 1/200,000

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

HSPD1 Tbio 60 kDa heat shock protein, mitochondrial (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
HSPD1 Tbio Heat shock protein HSP 60 (26 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 58 kDa
Exposure time: 15.0 s 

Hsp60 Mouse mAb (Ab169190) - Western Blot
All lanes: Hsp60 Mouse Antibody (Ab169190) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 4.0 s 

Hsp60 Mouse mAb (Ab169190) - Flow Cytometry
Overlay histogram showing Jurkat cells stained with Hsp60 Mouse mAb (Ab169190) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Hsp60 Mouse mAb (Ab169190) - ICC/IF
IF analysis of Hsp60 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (Ab169190) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/200

Flow Cytometry

1/800 - 1/200,000

WB

1/1,000 - 1/200,000; Predicted molecular weight: 61.0 KDa

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

5 results found

Lot Number Certificate Type Date Item
ZJ24F0809932 Certificate of Analysis Aug 27, 2024 Ab169190
ZJ24R0600698 Certificate of Analysis Jun 23, 2024 Ab169190
ZJ24F0606532 Certificate of Analysis Jun 05, 2024 Ab169190
ZJ24F0606531 Certificate of Analysis Jun 05, 2024 Ab169190
ZJ24F0606530 Certificate of Analysis Jun 05, 2024 Ab169190

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