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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab156228-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab156228-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab156228-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
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Ab156228-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
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mAb (CD05/2A2); Mouse anti Human Hsp27; WB, Flow Cytometry, IF/ICC, ELISA; Unconjugated
| Product Name | Hsp27 Mouse mAb - Primary antibody, specific to Hsp27 |
|---|---|
| Synonyms | Heat shock 27kDa protein antibody | 28 kDa heat shock protein antibody | CMT2F antibody | DKFZp586P1322 antibody | epididymis secretory protein Li 102 antibody | Estrogen regulated 24 kDa protein antibody | Estrogen-regulated 24 kDa protein antibody | Hea |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numer |
| Host species | Mouse |
| Specificity | Hsp27 |
| Immunogen | Recombinant protein corresponding to Human Hsp27 |
| Positive Control | WB: MCF-7, HeLa, K562, A431, A549 and LNCaP whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human Hsp27 Antibody, clone CD05/2A2, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.
|
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Hsp27 Mouse mAb (Ab156228) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab156228) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 7.6 s
Hsp27 Mouse mAb (Ab156228) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Hsp27 Mouse mAb (Ab156228) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).
Hsp27 Mouse mAb (Ab156228) - ICC/IF
IF analysis of Hsp27 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp27 Mouse mAb (Ab156228) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC | 1/200 |
| WB |
1/1000 - 1/20000
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| Flow Cytometry | 1/200 - 1/50000 |
Starting at $69.90
Starting at $66.90
Starting at $69.90