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Hsp27 Mouse mAb - Primary antibody, specific to Hsp27

    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2b
  • Clone Number: CD05/2A2
  • Conjugation: Unconjugated
In stock
Item Number
Ab156228
Grouped product items
SKU Size
Availability
Price Qty
Ab156228-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab156228-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab156228-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab156228-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (CD05/2A2); Mouse anti Human Hsp27; WB, Flow Cytometry, IF/ICC, ELISA; Unconjugated

Basic Description

Product Name Hsp27 Mouse mAb - Primary antibody, specific to Hsp27
Synonyms Heat shock 27kDa protein antibody | 28 kDa heat shock protein antibody | CMT2F antibody | DKFZp586P1322 antibody | epididymis secretory protein Li 102 antibody | Estrogen regulated 24 kDa protein antibody | Estrogen-regulated 24 kDa protein antibody | Hea
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numer
Host species Mouse
Specificity Hsp27
Immunogen Recombinant protein corresponding to Human Hsp27
Positive Control WB: MCF-7, HeLa, K562, A431, A549 and LNCaP whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human Hsp27 Antibody, clone CD05/2A2, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

HSPB1 Tchem Heat shock protein beta-1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Hsp27 Mouse mAb (Ab156228) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab156228) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 7.6 s 

Hsp27 Mouse mAb (Ab156228) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with Hsp27 Mouse mAb (Ab156228) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).​

Hsp27 Mouse mAb (Ab156228) - ICC/IF
IF analysis of Hsp27 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp27 Mouse mAb (Ab156228) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/200

WB

1/1000 - 1/20000

Flow Cytometry

1/200 - 1/50000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

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