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HMGB1 Mouse mAb - Primary antibody, specific to HMGB1, IgG2b

COA COA
    Application:
  • Flow Cytometry
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG2b
  • Clone Number: AB02/4A1
  • Conjugation: Unconjugated
In stock
Item Number
Ab156561
Grouped product items
SKU Size
Availability
 Price Qty
Ab156561-10μl
10μl
≥10
$66.90
Ab156561-20μl
20μl
6
$99.90
Ab156561-50μl
50μl
≥10
$199.90
Ab156561-100μl
100μl
≥10
$299.90
Ab156561-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,599.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (AB02/4A1); Mouse anti Human HMGB1 Antibody; WB, Flow, ELISA; Unconjugated

View related series
Click to view target information: HMGB1 Click to view target information: TCF7
Accession#:P09429 (4) Flow Cytometry (1158) Gene ID:3146 (4) HMGB1 (4)

Basic Description

Product Name HMGB1 Mouse mAb - Primary antibody, specific to HMGB1, IgG2b
Synonyms Amphoterin antibody | Chromosomal protein, nonhistone, HMG1 antibody | DKFZp686A04236 antibody | Heparin-binding protein p30 antibody | High mobility group 1 antibody | High mobility group box 1 antibody | High mobility group protein 1 antibody | High mob
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species Mouse
Specificity HMGB1
Immunogen Recombinant full length protein corresponding to Human HMGB1
Positive Control WB: HEK293, HeLa, HepG2, Jurkat, MCF-7, A549, A431, NIH/3T3 and C6 whole cell lysates Flow：HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1/2,000 - 1/100,000; Predicted molecular weight: 25.0 kDa
Flow: 0.02 μg/mL - 2.5 μg/mL

Product Properties

Isotype IgG2b
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

HMGB1 Tchem High mobility group protein B1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
TCF7 Tbio Transcription factor 7 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
HMGB1 Tchem High mobility group protein B1 (86 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

HMGB1 Mouse mAb (Ab156561) - Western Blot
All lanes: HMGB1 Antibody (Ab156561) at 0.1 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 24.9 kDa
Observed band size: 27.2 kDa
Exposure time: 118.1 s 

HMGB1 Mouse mAb (Ab156561) - Western Blot
All lanes: HMGB1 Antibody (Ab156561) at 0.1 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 24.9 kDa
Observed band size: 26.1 kDa
Exposure time: 27.8 s 

HMGB1 Mouse mAb (Ab156561) - ICC/IF
IF analysis of HMGB1 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HMGB1 Mouse mAb (Ab156561) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
WB

1/2,000 - 1/100,000; Predicted molecular weight: 25.0 kDa
Flow

0.02 μg/mL – 2.5 μg/mL
WB

1/2,000 - 1/50,000; Predicted molecular weight: 25.0 kDa
WB

1/2,000 - 1/100,000; Predicted molecular weight: 25.0 kDa
Flow Cytometry

0.02 μg/mL - 2.5 μg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

8 results found

Lot Number Certificate Type Date Item
ZJ24F0303596 Certificate of Analysis Apr 03, 2024 Ab156561
ZJ24F0303595 Certificate of Analysis Apr 03, 2024 Ab156561
ZJ24F0303594 Certificate of Analysis Apr 03, 2024 Ab156561
ZJ24F0303593 Certificate of Analysis Apr 03, 2024 Ab156561
ZJ24R0200300 Certificate of Analysis Mar 01, 2024 Ab156561
ZJ23F1202037 Certificate of Analysis Dec 12, 2023 Ab156561
ZJ23F1202036 Certificate of Analysis Dec 12, 2023 Ab156561
ZJ23F1202035 Certificate of Analysis Dec 12, 2023 Ab156561

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