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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab191623-10μg
|
10μg |
3
|
$31.90
|
|
|
Ab191623-25μg
|
25μg |
2
|
$63.90
|
|
|
Ab191623-100μg
|
100μg |
1
|
$139.90
|
|
mAb (W6/32); Mouse anti Human HLA Class I Antibody (Biotin); Flow Cytometry
| Product Name | HLA Class I Mouse mAb (Biotin) - Primary antibody, specific to HLA Class I, Mouse IgG2a |
|---|---|
| Synonyms | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I | HLAA | HLA-A | HLA-ABC | major histo |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Host species | Mouse |
| Specificity | HLA Class I |
| Immunogen | Human tonsil cell membrane |
| Positive Control | Flow: Human peripheral blood lymphocytes |
| Conjugation | Biotin |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Human HLA Class I Antibody, Monoclonal (W6/32), could be used for WB, Flow, IP, IHC, ICC/IF, Functional Assays and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| Light Chain Type | kappa |
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
HLA Class I Mouse mAb (Biotin) (Ab191623) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with HLA Class I Mouse mAb (Biotin) (Ab191623) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).
HLA Class I Mouse mAb (Biotin) (Ab191623) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with HLA Class I Mouse mAb (Biotin) (Ab191623) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume. |
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