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HLA Class I Mouse mAb (Biotin) - Primary antibody, specific to HLA Class I, Mouse IgG2a

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2a
  • Clone Number: W6/32
  • Conjugation: Biotin
In stock
Item Number
Ab191623
Grouped product items
SKU Size
Availability
Price Qty
Ab191623-10μg
10μg
3
$31.90
Ab191623-25μg
25μg
2
$63.90
Ab191623-100μg
100μg
1
$139.90

mAb (W6/32); Mouse anti Human HLA Class I Antibody (Biotin); Flow Cytometry

Basic Description

Product Name HLA Class I Mouse mAb (Biotin) - Primary antibody, specific to HLA Class I, Mouse IgG2a
Synonyms A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I | HLAA | HLA-A | HLA-ABC | major histo
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Host species Mouse
Specificity HLA Class I
Immunogen Human tonsil cell membrane
Positive Control Flow: Human peripheral blood lymphocytes
Conjugation Biotin
Grade ExactAb™, Validated
Product Description

Mouse anti Human HLA Class I Antibody, Monoclonal (W6/32), could be used for WB, Flow, IP, IHC, ICC/IF, Functional Assays and so on.

Product Properties

Isotype Mouse IgG2a
Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

HLA-A Tclin HLA class I histocompatibility antigen, A-3 alpha chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

HLA Class I Mouse mAb (Biotin) (Ab191623) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with HLA Class I Mouse mAb (Biotin) (Ab191623) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

HLA Class I Mouse mAb (Biotin) (Ab191623) - Flow Cytometry
Flow cytometry analysis of HLA Class I expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with HLA Class I Mouse mAb (Biotin) (Ab191623) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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