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Goat Anti-Rabbit IgG H&L (Biotin) - Secondary antibody

COA COA
    Application:
  • ELISA
  • IF/ICC
  • WB
  • Host species: Goat
  • Reactive species: Rabbit
  • Conjugation: Biotin
In stock
Item Number
Ab170143
Grouped product items
SKU Size
Availability
 Price Qty
Ab170143-100μg
100μg
5
$18.90
Ab170143-1mg
1mg
1
$109.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Secondary Antibody; Goat Anti-Rabbit IgG H&L (Biotin); WB, ELISA, IF

View related series
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Basic Description

Product Name Goat Anti-Rabbit IgG H&L (Biotin) - Secondary antibody
Synonyms Goat Anti-Rabbit IgG H&L Antibody (Biotin) | Anti-Rabbit IgG H&L Antibody (Biotin) | Rabbit IgG H&L Antibody (Biotin) | Biotin-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (Biotin) | Anti-Rabbit IgG Antibody (Biotin) | Rabbit IgG
Specifications & Purity ExactAb™, Azide Free, Validated, High Performance, Pre-adsorbed, 1.0 mg/mL
Host species Goat
Immunogen Rabbit IgG
Conjugation Biotin
Grade Azide Free, ExactAb™, High Performance, Pre-adsorbed, Validated
Concentration 1.0 mg/mL
Product Description

Biotin conjugated anti-rabbit secondary antibody is commonly used for immunohistochemistry, Western blotting, and ELISA.
Application
WB: 1:10000-1:200000
ELISA: 1:10000-1:100000
IF: 1:1000

Storage and Shipping

Shape Liquid
Storage Temp Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In Wet ice
Stability And Storage Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Antibody (Ab091004) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/2000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) - ELISA
Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) was tested by direct ELISA. The plate was coated with 100 ng of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (Biotin) (Ab170143) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Application

Application Dilution info
WB

1/10000-1/200000
IF/ICC

1:1000
ELISA

1:10000-1:100000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1001399 Certificate of Analysis Jul 30, 2024 Ab170143
ZJ23F1001400 Certificate of Analysis Jul 30, 2024 Ab170143

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