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Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody

1 Citations COA COA
    Application:
  • ELISA
  • IF/ICC
  • WB
  • Host species: Goat
  • Reactive species: Mouse
  • Conjugation: Biotin
In stock
Item Number
Ab156253
Grouped product items
SKU Size
Availability
 Price Qty
Ab156253-100μg
100μg
5
$19.90
Ab156253-1mg
1mg
1
$99.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Secondary Antibody; Goat Anti-Mouse IgG H&L (Biotin); WB, ELISA, IF, Flow

View related series
CLARITY tissue clearing technology (64) Goat Anti-Mouse (14)

Basic Description

Product Name Goat Anti-Mouse IgG H&L (Biotin) - Secondary antibody
Synonyms Goat Anti-Mouse IgG H&L Antibody (Biotin) | Anti-Mouse IgG H&L Antibody (Biotin) | Mouse IgG H&L Antibody (Biotin) | Biotin-conjugated Goat Anti-Mouse IgG (H+L) | Goat Anti Mouse IgG Antibody (Biotin) | Anti-Mouse IgG Antibody (Biotin) | Mouse IgG Antibod
Specifications & Purity ExactAb™, Azide Free, Validated, High Performance, Pre-adsorbed, 1.0 mg/mL
Host species Goat
Immunogen Mouse IgG
Positive Control ELISA: Mouse IgG
Conjugation Biotin
Grade Azide Free, ExactAb™, High Performance, Pre-adsorbed, Validated
Purification Method Antigen affinity purified
Concentration 1.0 mg/mL
Product Description

Application
WB: 1:10,000-1:200,000
ELISA: 1:20,000-1:400,000
IF: 1:1000

Storage and Shipping

Shape Liquid
Storage Temp Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In Wet ice
Stability And Storage Store at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) - ELISA
Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was tested by direct ELISA. The plate was coated with different amounts of Mouse IgG (Ab170221). A serial dilution of Goat Anti-Mouse IgG H&L (Biotin) (Ab156253) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Application

Application Dilution info
WB

1/10000-1/200000
IF/ICC

1:1000
ELISA

1/20000-1/400000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1001199 Certificate of Analysis Jul 30, 2024 Ab156253
ZJ23F1001198 Certificate of Analysis Jul 30, 2024 Ab156253

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