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Fibrinogen gamma chain Mouse mAb - Primary antibody, specific to Fibrinogen gamma chain, Mouse IgG1

    Application:
  • IHC
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: K24024_2E1
  • Conjugation: Unconjugated
In stock
Item Number
Ab103501
Grouped product items
SKU Size
Availability
Price Qty
Ab103501-10μl
10μl
2
$69.90
Ab103501-50μl
50μl
1
$199.90
Ab103501-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab103501-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

mAb (K24024_2E1); Mouse anti Human Fibrinogen gamma chain Antibody; WB, IHC, IP; Unconjugated

Basic Description

Product Name Fibrinogen gamma chain Mouse mAb - Primary antibody, specific to Fibrinogen gamma chain, Mouse IgG1
Synonyms FGG antibody | FIBG_HUMAN antibody | Fibrinogen gamma chain antibody | Fibrinogen gamma polypeptide antibody | fibrinogen gamma-b chain antibody
Specifications & Purity Carrier Free, ExactAb™, Validated, 1.0 mg/mL
Host species Mouse
Specificity Fibrinogen gamma chain
Immunogen Recombinant protein of human FGG (AA 61-160)
Positive Control WB: HepG2 lysates. IHC: Human kidney tissue. IP: HepG2 lysates.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human Fibrinogen gamma chain Antibody, Monoclonal (K24024_2E1), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/500
IP: 1/100 
Protein Function:  
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -80°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

FGG Tbio Fibrinogen gamma chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Fibrinogen gamma chain Mouse mAb (Ab103501) - Western Blot
All lanes: Fibrinogen gamma chain Mouse mAb (Ab103501) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 30.0 s

Fibrinogen gamma chain Mouse mAb (Ab103501) - IHC
IHC analysis of kidney tissue by Fibrinogen gamma chain Mouse mAb (Ab103501).IHC was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with Fibrinogen gamma chain Mouse mAb (Ab103501) at 2 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in glomeruli are positively stained at the cytoplasm.

Fibrinogen gamma chain Mouse mAb (Ab103501) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of Fibrinogen gamma chain Mouse mAb (Ab103501) with Hep G2 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Fibrinogen gamma chain Mouse mAb (Ab103501) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: Hep G2 lysateLane 2: FGG immunoprecipitated from Hep G2 lysate by Fibrinogen gamma chain Mouse mAb (Ab103501)
Lane 3: The isotype control antibody instead of Fibrinogen gamma chain Mouse mAb (Ab103501) in Hep G2 lysate. Result: Fibrinogen gamma chain Mouse mAb (Ab103501) can immunoprecipitate FGG.

Application

Application Dilution info
WB

1/1000

IP

1/100

IHC

1/500

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0708179 Certificate of Analysis Jul 11, 2024 Ab103501
ZJ24F0708180 Certificate of Analysis Jul 11, 2024 Ab103501

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