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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab103501-10μl
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10μl |
2
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$69.90
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Ab103501-50μl
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50μl |
1
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$199.90
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Ab103501-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
|
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Ab103501-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,599.90
|
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mAb (K24024_2E1); Mouse anti Human Fibrinogen gamma chain Antibody; WB, IHC, IP; Unconjugated
| Product Name | Fibrinogen gamma chain Mouse mAb - Primary antibody, specific to Fibrinogen gamma chain, Mouse IgG1 |
|---|---|
| Synonyms | FGG antibody | FIBG_HUMAN antibody | Fibrinogen gamma chain antibody | Fibrinogen gamma polypeptide antibody | fibrinogen gamma-b chain antibody |
| Specifications & Purity | Carrier Free, ExactAb™, Validated, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | Fibrinogen gamma chain |
| Immunogen | Recombinant protein of human FGG (AA 61-160) |
| Positive Control | WB: HepG2 lysates. IHC: Human kidney tissue. IP: HepG2 lysates. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human Fibrinogen gamma chain Antibody, Monoclonal (K24024_2E1), could be used for WB, IHC, IP and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Fibrinogen gamma chain Mouse mAb (Ab103501) - Western Blot
All lanes: Fibrinogen gamma chain Mouse mAb (Ab103501) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 30.0 s
Fibrinogen gamma chain Mouse mAb (Ab103501) - IHC
IHC analysis of kidney tissue by Fibrinogen gamma chain Mouse mAb (Ab103501).IHC was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with Fibrinogen gamma chain Mouse mAb (Ab103501) at 2 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in glomeruli are positively stained at the cytoplasm.
Fibrinogen gamma chain Mouse mAb (Ab103501) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of Fibrinogen gamma chain Mouse mAb (Ab103501) with Hep G2 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Fibrinogen gamma chain Mouse mAb (Ab103501) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: Hep G2 lysateLane 2: FGG immunoprecipitated from Hep G2 lysate by Fibrinogen gamma chain Mouse mAb (Ab103501)
Lane 3: The isotype control antibody instead of Fibrinogen gamma chain Mouse mAb (Ab103501) in Hep G2 lysate. Result: Fibrinogen gamma chain Mouse mAb (Ab103501) can immunoprecipitate FGG.
| Application | Dilution info |
|---|---|
| WB |
1/1000
|
| IP | 1/100 |
| IHC | 1/500 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Jul 11, 2024 | Ab103501 | |
| Certificate of Analysis | Jul 11, 2024 | Ab103501 |