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DDX3 Mouse mAb - Primary antibody, specific to DDX3, Mouse IgG2a

    Application:
  • IF/ICC
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Human,Monkey,Mouse,Rat
  • Isotype: Mouse IgG2a
  • Clone Number: 6G8-F4-E3
  • Conjugation: Unconjugated
In stock
Item Number
Ab099443
Grouped product items
SKU Size
Availability
Price Qty
Ab099443-10μl
10μl
2
$69.90
Ab099443-50μl
50μl
1
$199.90
Ab099443-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab099443-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,499.90

mAb (6G8-F4-E3); Mouse anti Human DDX3 Antibody; WB, ICC, IF, IP; Unconjugated

Basic Description

Product Name DDX3 Mouse mAb - Primary antibody, specific to DDX3, Mouse IgG2a
Synonyms ATP dependent RNA helicase DDX3X antibody | ATP-dependent RNA helicase DDX3X antibody | CAP Rf antibody | DBX antibody | DDX14 antibody | DDX3X antibody | DDX3X_HUMAN antibody | DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody | DEAD (Asp-Glu-Al
Specifications & Purity ExactAb™, Validated, 1.0 mg/mL
Host species Mouse
Specificity DDX3
Immunogen A synthetic peptide derived from human DDX3 (AA 22-178)
Positive Control WB: Jurkat, K562, HeLa, A431, HEK293, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. IP: HeLa lysates.
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Mouse anti Human DDX3 Antibody, Monoclonal (6G8-F4-E3), could be used for WB, ICC, IF, IP and so on.
Application
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
IP: 1/20
Protein Function
ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.

Product Properties

Isotype Mouse IgG2a
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

DDX3X Tchem ATP-dependent RNA helicase DDX3X (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

DDX3 Mouse mAb (Ab099443) - Western Blot
All lanes: DDX3 Mouse mAb (Ab099443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 75 kDa
Exposure time: 18.7 s

DDX3 Mouse mAb (Ab099443) - IF/ICC
Immunocytochemistry analysis of DDX3 (6G8) in HeLa using DDX3 Mouse mAb (Ab099443).

DDX3 Mouse mAb (Ab099443) - IP
Immunoprecipitation analysis of DDX3 in HeLa lysates using DDX3 Mouse mAb (Ab099443).

Application

Application Dilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

IP

1/20

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0505897 Certificate of Analysis May 21, 2024 Ab099443
ZJ24F0505896 Certificate of Analysis May 21, 2024 Ab099443

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