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CDK5 Mouse mAb - Primary antibody, specific to CDK5, Mouse IgG1

    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 1552CT262.105.8
  • Conjugation: Unconjugated
In stock
Item Number
Ab095769
Grouped product items
SKU Size
Availability
Price Qty
Ab095769-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab095769-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$199.90
Ab095769-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$299.90
Ab095769-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,099.90

mAb(1552CT262.105.8); Mouse anti human CDK5 A ntibody; WB, IHC, Flow, IF, ICC; Unconjugated

Basic Description

Product Name CDK5 Mouse mAb - Primary antibody, specific to CDK5, Mouse IgG1
Synonyms Cdk 5 antibody | Cdk5 antibody | CDK5_HUMAN antibody | Cell division protein kinase 5 antibody | Crk6 antibody | Cyclin dependent kinase 5 antibody | Cyclin-dependent kinase 5 antibody | Protein kinase CDK5 splicing antibody | PSSALRE antibody | Serine th
Specifications & Purity ExactAb™, Validated, Carrier Free, 0.5mg/mL
Host species Mouse
Specificity CDK5
Immunogen A recombinant protein of human CDK5 ( AA 1-292).
Positive Control WB: A431, K562, HeLa, NIH/3T3, A549, Jurkat, MCF7 and HEK293 cell lysates. IHC: Human skeletal muscle tissue. IF/ICC: A549 cells. Flow: K562 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti human CDK5 Antibody, Monoclonal (1552CT262.105.8), could be used for WB, IHC, Flow, ICC, IF and so on.
Applications
WB: 1/2000
IHC: 1/100-1/500
IF/ICC: 1/25
Flow:1/25
Function
Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

CDK5 Tchem Cyclin-dependent-like kinase 5 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
CDK5 Tchem Cyclin-dependent kinase 5 (3021 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
CDK5 Tchem Cyclin-dependent kinase 5/CDK5 activator 1 (3697 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
CDK5 Tchem Cyclin-T1/Cyclin-dependent-like kinase 5 (5 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CDK5 Mouse mAb (Ab095769) - Western Blot
All lanes: CDK5 Mouse mAb (Ab095769) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 11.9 s

CDK5 Mouse mAb (Ab095769) - ICC/IF
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling CDK5 with CDK5 Mouse mAb (Ab095769) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

CDK5 Mouse mAb (Ab095769) - IHC
CDK5 Mouse mAb (Ab095769) staining CDK5 in human skeletal muscle sections by Immunohistochemistry (paraformaldehyde - fixed, paraffin - embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

CDK5 Mouse mAb (Ab095769) - Flow Cytometry
Overlay histogram showing K562 cells stained with CDK5 Mouse mAb (Ab095769) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by CDK5 Mouse mAb (Ab095769) (1/25 dilution) for 60 min at 37ºC. The secondary antibody was used Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 used under the same conditions. Acquisition of >10000 events was performed.

Application

Application Dilution info
IHC

1/100-1/500

Flow Cytometry

1/25

WB

1/2000

IF/ICC

1/25

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1001320 Certificate of Analysis Oct 18, 2023 Ab095769
ZJ23F1001319 Certificate of Analysis Oct 18, 2023 Ab095769

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