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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab095769-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$66.90
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Ab095769-50μl
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50μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
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$199.90
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Ab095769-100μl
|
100μl |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
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$299.90
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Ab095769-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,099.90
|
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mAb(1552CT262.105.8); Mouse anti human CDK5 A ntibody; WB, IHC, Flow, IF, ICC; Unconjugated
| Product Name | CDK5 Mouse mAb - Primary antibody, specific to CDK5, Mouse IgG1 |
|---|---|
| Synonyms | Cdk 5 antibody | Cdk5 antibody | CDK5_HUMAN antibody | Cell division protein kinase 5 antibody | Crk6 antibody | Cyclin dependent kinase 5 antibody | Cyclin-dependent kinase 5 antibody | Protein kinase CDK5 splicing antibody | PSSALRE antibody | Serine th |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5mg/mL |
| Host species | Mouse |
| Specificity | CDK5 |
| Immunogen | A recombinant protein of human CDK5 ( AA 1-292). |
| Positive Control | WB: A431, K562, HeLa, NIH/3T3, A549, Jurkat, MCF7 and HEK293 cell lysates. IHC: Human skeletal muscle tissue. IF/ICC: A549 cells. Flow: K562 cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti human CDK5 Antibody, Monoclonal (1552CT262.105.8), could be used for WB, IHC, Flow, ICC, IF and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 0.5mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
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CDK5 Mouse mAb (Ab095769) - Western Blot
All lanes: CDK5 Mouse mAb (Ab095769) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution
Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 11.9 s
CDK5 Mouse mAb (Ab095769) - ICC/IF
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling CDK5 with CDK5 Mouse mAb (Ab095769) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
CDK5 Mouse mAb (Ab095769) - IHC
CDK5 Mouse mAb (Ab095769) staining CDK5 in human skeletal muscle sections by Immunohistochemistry (paraformaldehyde - fixed, paraffin - embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
CDK5 Mouse mAb (Ab095769) - Flow Cytometry
Overlay histogram showing K562 cells stained with CDK5 Mouse mAb (Ab095769) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by CDK5 Mouse mAb (Ab095769) (1/25 dilution) for 60 min at 37ºC. The secondary antibody was used Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 used under the same conditions. Acquisition of >10000 events was performed.
| Application | Dilution info |
|---|---|
| IHC | 1/100-1/500 |
| Flow Cytometry | 1/25 |
| WB | 1/2000 |
| IF/ICC | 1/25 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Oct 18, 2023 | Ab095769 | |
| Certificate of Analysis | Oct 18, 2023 | Ab095769 |