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Mouse IgG2b-kappa, anti-E8L - Isotype Control Antibody, specific to E8L

COA COA
    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • Host species: Mouse
  • Reactive species: Virus
  • Isotype: Mouse IgG2b
  • Clone Number: EF11/6E10
  • Conjugation: Unconjugated
In stock
Item Number
Ab210600
Grouped product items
SKU Size
Availability
 Price Qty
Ab210600-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$19.90
Ab210600-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab210600-5mg
5mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$279.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (EF11/6E10); Mouse IgG2b Isotype Control Antibody; Flow Cytometry, IF/ICC, ELISA; Unconjugated

View related series
Accession#:Q8V4Y0 (28) E8L (28) Flow Cytometry (1158) Gene ID:929017 (28) Mouse IgG2b-kappa Isotype Control (14)

Basic Description

Product Name Mouse IgG2b-kappa, anti-E8L - Isotype Control Antibody, specific to E8L
Synonyms Mouse IgG2b, κ Isotype Control | Purified Mouse IgG2b, kappa Isotype Control | Mouse IgG2b Isotype Control Antibody | Mouse IgG2b Isotype Control mAb | Immunoglobulin G2B Fc | Mouse IgG2B Isotype Control | Isotype control mAb for mouse IgG2b | IgG2b (Mous
Specifications & Purity Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two he
Host species Mouse
Specificity E8L
Immunogen Recombinant protein corresponding to MPXV E8L
Positive Control Flow Cytometry: Peripheral blood mononuclear cells; Mouse splenocytes IF/ICC: HeLa cells
Conjugation Unconjugated
Grade Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse IgG2b, Isotype Control Antibody (EF11/6E10), could be used for Flow Cytometry analysis, IF/ICC, ELISA and so on.  

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Mouse IgG2b-kappa, anti-E8L (Ab210600) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood mononuclear cells labelling CD45RA (Red) with Recombinant CD45RA Antibody (Ab212152) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, compared to concentration matched Mouse IgG2b-kappa, anti-E8L (Ab210600) (Blue) conjugated with biotin. Detected with SA-PE (rp156150).

Mouse IgG2b-kappa, anti-E8L (Ab210600) - Flow Cytometry
Flow Cytometry analysis of C57BL/6 Mouse splenocytes labelling Mouse IgG2b (Left panel) with Mouse IgG2b-kappa, anti-E8L (Ab210600) conjugated with biotin at 10.0 μg/mL for 1 hour at 4°C, compared with unlabelled control (Right panel). Detected with SA-PE (rp156150).

Mouse IgG2b-kappa, anti-E8L (Ab210600) - ICC/IF
IF analysis of HeLa cells using beta Tubulin Mouse mAb (Ab179922) (Green) compared to concentration matched Mouse IgG2b-kappa, anti-E8L (Ab210600). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. A Goat Anti-Mouse IgG H&L (FITC) (Ab179003) was used at a dilution of 1/1000. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
Flow Cytometry

Use at the same concentration as the detection antibody.
IF/ICC

Use at the same concentration as the detection antibody.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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