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Transferrin Receptor/CD71 Mouse mAb - Primary antibody, specific to Transferrin Receptor/CD71, Mouse IgG1

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Transferrin Receptor/CD71 Mouse mAb - Primary antibody, specific to Transferrin Receptor/CD71, Mouse IgG1

Grade & Purity:

≥95%(SDS-PAGE)
See COA

Application:

Flow Cytometry
IF/ICC
IHC

Host species: Mouse
Reactive species: Human
Isotype: Mouse IgG1
Clone Number: OKT-9
Conjugation: Unconjugated

In stock

Item Number

Ab132254

Grouped product items
SKU	Size	Availability	Price	Qty
Ab132254-10μg	10μg	10	$29.90
Ab132254-50μg	50μg	4	$69.90
Ab132254-100μg	100μg	2	$109.90
Ab132254-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$899.90

mAb (OKT-9); Mouse anti Human Transferrin Receptor/CD71 Antibody; IHC, ICC, IF, Flow; Unconjugated

View related series

Click to view target information: TFRC

Accession#: P02786 (14) CD71 (21) Flow Cytometry (1158) Gene ID: 7037 (14) Transferrin Receptor (8)

Basic Description

Product Name	Transferrin Receptor/CD71 Mouse mAb - Primary antibody, specific to Transferrin Receptor/CD71, Mouse IgG1
Synonyms	TFR \| TRFR \| IMD46 \| CD71 antigen \| CD71 \| CD71TRFR \| p90 \| T9 \| TfR (Transferrin R) \| TfR (TransferrinR) \| TfR \| TFR1 \| TFRC \| TR \| transferrin receptor (p90, CD71) \| transferrin receptor protein 1 \| CD 71 antibody \| CD71 antibody \| CD71 antigen antibody
Specifications & Purity	Carrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species	Mouse
Specificity	Transferrin Receptor/CD71
Immunogen	Human acute lymphoblastic leukemia cells
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, Validated
Product Description	Mouse anti Human Transferrin Receptor/CD71 Antibody, Monoclonal (OKT-9), could be used for IHC, ICC, IF, Flow and so on. Application: Flow: Use at an assay dependent concentration. IHC: Use at an assay dependent concentration. IF/ICC: Use at an assay dependent concentration. Protein Function Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

Product Properties

Isotype	Mouse IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at 2-8°C,Do not freeze
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

TFRC Tclin Transferrin receptor protein 1 (0 Activities)

Discover Target: TFRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

TFRC Tclin Transferrin receptor protein 1 (0 Activities)

Discover Target: TFRC

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated Jurkat cells (24h) labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of MOLT-4 cells labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 5.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated MOLT-4 cells (24h) labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 2.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (Ab132254)

Transferrin Receptor/CD71 Mouse mAb (Ab132254) - Flow Cytometry
Flow Cytometry analysis of U937 cells labelling Transferrin Receptor/CD71 (red) with Transferrin Receptor/CD71 Mouse mAb (Ab132254) at 2.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
IHC	Use at an assay dependent concentration.
Flow Cytometry	Use at an assay dependent concentration.
IF/ICC	Use at an assay dependent concentration.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0707977	Certificate of Analysis	Jul 09, 2024	Ab132254
ZJ24F0707978	Certificate of Analysis	Jul 09, 2024	Ab132254
ZJ24F0707979	Certificate of Analysis	Jul 09, 2024	Ab132254

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