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Recombinant CD45 Antibody (PE-Cy5), specific to CD45

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: P51B13
  • Conjugation: PE-Cy5
In stock
Item Number
Ab213121
Grouped product items
SKU Size
Availability
Price Qty
Ab213121-10T
10T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$56.90
Ab213121-25T
25T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$111.90
Ab213121-100T
100T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$269.90

Recombinant (P51B13); Mouse anti Human CD45 Antibody (PE-Cy5); Flow Cytometry

Basic Description

Product Name Recombinant CD45 Antibody (PE-Cy5), specific to CD45
Synonyms B220 | CD45 | CD45 antigen | CD45R | EC 3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase | PTPRC | receptor-type tyrosine-protein phosphatase C | T200 Glycoprotein | T200 leukocyte common antigen | T200 | B220
Specifications & Purity ExactAb™, Validated, Ex:565nm, Em:670nm, 5 μL/test
Biochemical and Physiological Mechanisms Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second
Host species Mouse
Specificity CD45
Immunogen Peripheral blood mononuclear cells
Positive Control Flow: Human peripheral blood lymphocytes
Conjugation PE-Cy5
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 670nm
Ex/Em(nm) Ex:565nm;Em:670nm
Grade Em:670nm, Ex:565nm, ExactAb™, Validated
Product Description

Recombinant CD45 Antibody, clone P51B13, could be used for Flow Cytometry and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Concentration 5 μL/test
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant CD45 Antibody (PE-Cy5) (Ab213121) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45 Antibody (PE-Cy5) (Ab213121) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy5) (Ab190078).

Recombinant CD45 Antibody (PE-Cy5) (Ab213121) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (PE-Cy5) (Ab213121) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy5) (Ab190078). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot Number Certificate Type Date Item
ZJ24R1201604 Certificate of Analysis Dec 21, 2024 Ab213121

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