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CD86 Rat mAb - Primary antibody, specific to CD86, Rat IgG2a

COA COA
    Application:
  • Animal Model
  • Blocking
  • ELISA
  • Flow Cytometry
  • Functional Assay
  • IHC
  • IHC-F
  • IP
  • WB
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: GL1
  • Conjugation: Unconjugated
In stock
Item Number
Ab095462
Grouped product items
SKU Size
Availability
 Price Qty
Ab095462-10μg
10μg
10
$16.90
Ab095462-50μg
50μg
4
$49.90
Ab095462-100μg
100μg
2
$89.90
Ab095462-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$519.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (GL1); Rat anti Mouse CD86 Antibody; Flow Cytometry, WB, IHC-F, IP, Blocking, ELISA, Functional Assay, Animal Model; Unconjugated

View related series
Click to view target information: CD86
Accession#:P42082 (13) CD86 (17) Flow Cytometry (1158) Gene ID:12524 (13)

Basic Description

Product Name CD86 Rat mAb - Primary antibody, specific to CD86, Rat IgG2a
Synonyms Activation B7-2 antigen | B70 | B7-2 antigen | B72 | B7-2 | B-lymphocyte activation antigen B7-2 | BU63 | CD28 antigen ligand 2 | CD28LG2B7-2 antigen) | CD86 antigen | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | FUN-1 | LAB72 | MGC34413 | T-lymp
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, See COA
Biochemical and Physiological Mechanisms Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d
Host species Rat
Specificity CD86
Immunogen LPS-activated CBA/Ca mouse splenic B cells
Positive Control Flow: Mouse splenocytes.
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, Validated
Product Description

 Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry, WB, IHC-F, IP, Blocking, ELISA, Functional Assay, Animal Model and so on.

Product Properties

Isotype Rat IgG2a
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at 2-8°C,Do not freeze
Shipped In Wet ice
Stability And Storage Store at 2-8°C. Store at -20°C for long term. Upon delivery aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD86 Rat mAb (Ab095462) - Flow Cytometry
Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 (red) with CD86 Rat mAb (Ab095462) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

CD86 Rat mAb (Ab095462) - Flow Cytometry
Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 with CD86 Rat mAb (Ab095462) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156150).

Application

Application Dilution info
Flow Cytometry

0.25μg/100w cells
WB

Use at an assay dependent concentration.
Blocking

Use at an assay dependent concentration.
Functional Assay

Use at an assay dependent concentration.
IP

Use at an assay dependent concentration.
ELISA

Use at an assay dependent concentration.
Animal Model

Use at an assay dependent concentration. 
IHC-F

Use at an assay dependent concentration.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0505481 Certificate of Analysis May 13, 2024 Ab095462
ZJ24F0505480 Certificate of Analysis May 13, 2024 Ab095462
ZJ24F0505479 Certificate of Analysis May 13, 2024 Ab095462

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