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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab185407-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$15.90
|
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Ab185407-25μg
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25μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$29.90
|
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Ab185407-100μg
|
100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
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mAb (17A2); Rat anti Mouse CD3 Antibody (PE); Flow Cytometry
| Product Name | CD3 Rat mAb (PE) - Primary antibody, specific to CD3 |
|---|---|
| Synonyms | CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | The CD3 complex mediates signal transduction. |
| Host species | Rat |
| Specificity | CD3 |
| Immunogen | γδTCR-positive T-T hybridoma D1 |
| Positive Control | Flow Cytometry: C57BL/6 Mouse splenocytes |
| Conjugation | PE |
| Excitation(nm) | Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm) |
| Emission(nm) | 575nm |
| Ex/Em(nm) | Ex:565nm;Em:575nm |
| Grade | ExactAb™, Validated |
| Product Description |
Rat anti Mouse CD3 Antibody, Monoclonal (17A2), could be used for Flow Cytometry. |
| Light Chain Type | kappa |
|---|---|
| Purification Method | Protein G purified |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
CD3 Rat mAb (PE) (Ab185407) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Rat mAb (PE) (Ab185407) at 1.0μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).
CD3 Rat mAb (PE) (Ab185407) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD3 Rat mAb (PE) (Ab185407) at 1.0μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | ≤1.0 μg/test |
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