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CD3 Rat mAb (PE) - Primary antibody, specific to CD3

    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2b
  • Clone Number: 17A2
  • Conjugation: PE
In stock
Item Number
Ab185407
Grouped product items
SKU Size
Availability
Price Qty
Ab185407-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$15.90
Ab185407-25μg
25μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$29.90
Ab185407-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90

mAb (17A2); Rat anti Mouse CD3 Antibody (PE); Flow Cytometry

Basic Description

Product Name CD3 Rat mAb (PE) - Primary antibody, specific to CD3
Synonyms CD_antigen: CD3e | CD3 antigen, delta subunit | CD3 | CD3d antigen | CD3d antigen, delta polypeptide (TiT3 complex) | CD3d molecule, delta (CD3-TCR complex) | CD3-DELTA | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, eps
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms The CD3 complex mediates signal transduction.
Host species Rat
Specificity CD3
Immunogen γδTCR-positive T-T hybridoma D1
Positive Control Flow Cytometry: C57BL/6 Mouse splenocytes
Conjugation PE
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 575nm
Ex/Em(nm) Ex:565nm;Em:575nm
Grade ExactAb™, Validated
Product Description

Rat anti Mouse CD3 Antibody, Monoclonal (17A2), could be used for Flow Cytometry.

Product Properties

Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD3G Tclin T-cell surface glycoprotein CD3 gamma chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

CD3 Rat mAb (PE) (Ab185407) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Rat mAb (PE) (Ab185407) at 1.0μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

CD3 Rat mAb (PE) (Ab185407) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD3 Rat mAb (PE) (Ab185407) at 1.0μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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