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CD3 Mouse mAb (AF488) - Primary antibody, specific to CD3, Mouse IgG1

COA COA
    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: UCHT1
  • Conjugation: AF488
In stock
Item Number
Ab189661
Grouped product items
SKU Size
Availability
 Price Qty
Ab189661-10T
10T
5
$53.90
Ab189661-25T
25T
5
$106.90
Ab189661-100T
100T
5
$259.90
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Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (UCHT1); Mouse anti Human CD3 Antibody (AF488); Flow

View related series
Click to view target information: CD3E
Accession#:P07766 (33) AF488 (72) CD3E (48) CD3epsilon (13) Flow Cytometry (1158) Gene ID:916 (29) IMD18 (11) T3E (19) TCRE (12)

Basic Description

Product Name CD3 Mouse mAb (AF488) - Primary antibody, specific to CD3, Mouse IgG1
Synonyms CD3epsilon | IMD18 | CD3 epsilon | CD3e antigen | CD3e antigen, epsilon polypeptide (TiT3 complex) | CD3e molecule, epsilon (CD3-TCR complex) | CD3e | CD3-epsilon | FLJ18683 | T3E | T-cell antigen receptor complex, epsilon subunit of T3 | T-cell surface a
Specifications & Purity ExactAb™, Validated, Ex:490nm, Em:525nm, 5 μL/test
Biochemical and Physiological Mechanisms The CD3 complex mediates signal transduction.
Host species Mouse
Specificity CD3
Immunogen Human CD3ε
Positive Control Flow: Human peripheral blood lymphocytes
Conjugation AF488
Excitation(nm) Blue Laser(488nm)
Emission(nm) 525nm
Ex/Em(nm) Ex:490nm;Em:525nm
Grade Em:525nm, Ex:490nm, ExactAb™, Validated
Product Description

Mouse anti Human CD3 Antibody, Monoclonal (UCHT1), could be used for Flow, in vivo T cell depletion in humanized mice, ex vivo T cell inhibition for xenographs and so on.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
Purification Method Protein G purified
Concentration 5 μL/test
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD3 Mouse mAb (AF488) (Ab189661) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD3 Mouse mAb (AF488) (Ab189661) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).

CD3 Mouse mAb (AF488) (Ab189661) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD3 Mouse mAb (AF488) (Ab189661) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245).Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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