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BRG1 Mouse mAb - Primary antibody, specific to BRG1, Mouse IgG1

COA COA
    Application:
  • IF/ICC
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse
  • Isotype: Mouse IgG1
  • Clone Number: 6D7-F7-B6
  • Conjugation: Unconjugated
In stock
Item Number
Ab091716
Grouped product items
SKU Size
Availability
 Price Qty
Ab091716-10μl
10μl
5
$69.90
Ab091716-50μl
50μl
2
$209.90
Ab091716-100μl
100μl
1
$319.90
Ab091716-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (6D7-F7-B6); Mouse anti Human BRG1 Antibody; WB, ICC, IF, IP; Unconjugated

View related series
Click to view target information: SMARCA4
Accession#:P51532 (1) BRG1 (1) Gene ID:6597 (1) SMARCA4 (1)

Basic Description

Product Name BRG1 Mouse mAb - Primary antibody, specific to BRG1, Mouse IgG1
Synonyms ATP-dependent helicase SMARCA4 | BAF190 | BAF190A | brahma protein-like 1 | Brg1 | BRG1-associated factor 190A | BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4 | BRM/SWI2-related gene 1 | EC 3.6.1 | EC 3.6
Specifications & Purity ExactAb™, Validated, See COA
Host species Mouse
Specificity BRG1
Immunogen Purified recombinant human BRG1 protein fragments expressed in E.coli (AA 205-300).
Positive Control WB: HeLa, Raw264.7, K562, Jurkat and Hep G2 cell lysates. ICC/IF: HeLa cells. IP: K562 cell lysates.
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Mouse anti Human BRG1 Antibody, Monoclonal (6D7-F7-B6), could be used for WB, ICC, IF, IP and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
IP: 1/20
Protein Function
Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

SMARCA4 Tchem Transcription activator BRG1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
SMARCA4 Tchem Transcription activator BRG1 (263 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

BRG1 Mouse mAb (Ab091716) - Western Blot
All lanes: BRG1 Mouse mAb (Ab091716) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 220 kDa
Observed band size: 213 kDa
Exposure time: 61.7 s

BRG1 Mouse mAb (Ab091716) - IF
Immunocytochemistry analysis of BRG1 (green) in HeLa cells using BRG1 Mouse mAb (Ab091716) and cells were counterstained with DAPI (blue).

BRG1 Mouse mAb (Ab091716) - IP
Immunoprecipitation analysis of BRG1 in K562 cell lysates using BRG1 antibody.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IP

1/20

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0901059 Certificate of Analysis Sep 14, 2023 Ab091716
ZJ23F0901058 Certificate of Analysis Sep 14, 2023 Ab091716

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