Basic Description

Product Name	beta Actin Mouse mAb - Primary antibody, for WB,Flow,ICC/IF,ELISA various applications, from Human, generated in Mouse, specific to beta actin, IgG1
Synonyms	A26C1A antibody \| A26C1B antibody \| ACTB antibody \| ACTB_HUMAN antibody \| Actin beta antibody \| Actin cytoplasmic 1 antibody \| Actin, cytoplasmic 1, N-terminally processed antibody \| Actx antibody \| b actin antibody \| b-actin antibody \| Beta cytoskeletal
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host Species	Mouse
Host species	Mouse
Specificity	beta actin
Application	WB,Flow,ICC/IF,ELISA
Immunogen	Synthetic peptide in beta Actin
Positive Control	WB: A549, MCF-7, HeLa, Hep G2, Raji, Ramos, MOLT-4, Jurkat, LNCap, A431, HEK293, NIH/3T3, K562, HT-29, THP-1 lysates. ICC/IF: HeLa cells. Flow: HeLa cells.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description	Application： WB: 1/1,000-1/20,000; Predicted molecular weight: 42 kDa. Flow: 0.016-2.0μg/mL ICC/IF: 0.5μg/mL Protein Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Product Properties

Antibody Type	Primary antibody
Clonality	Monoclonal antibodies
Isotype	IgG1
Light Chain Type	kappa
Purification Method	Protein A purified
Source	Hybridoma supernatant
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ACTB Tbio Actin, cytoplasmic 1 (0 Activities)

Discover Target: ACTB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: beta Actin Mouse mAb (Ab155822)

beta Actin Mouse mAb (Ab155822) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

ICC/IF: beta Actin Mouse mAb (Ab155822)

beta Actin Mouse mAb (Ab155822) - ICC/IF
IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: beta Actin Mouse mAb (Ab155822)

beta Actin Mouse mAb (Ab155822) - Flow Cytometry
Flow analysis of beta Actin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Application

Application	Dilution info
IF/ICC	0.5 μg/mL
Flow Cytometry	0.016-2.0μg/mL
WB	1/1,000-1/20,000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

7 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0700609	Certificate of Analysis	Apr 25, 2025	Ab155822
ZJ24F0404950	Certificate of Analysis	Apr 26, 2024	Ab155822
ZJ24F0404949	Certificate of Analysis	Apr 26, 2024	Ab155822
ZJ24F0404948	Certificate of Analysis	Apr 26, 2024	Ab155822
ZJ24F0404947	Certificate of Analysis	Apr 26, 2024	Ab155822
ZJ23R0900103	Certificate of Analysis	Sep 19, 2023	Ab155822
ZJ23F0700608	Certificate of Analysis	Jul 14, 2023	Ab155822

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