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beta 2 Microglobulin Mouse mAb - Primary antibody, specific to beta 2 Microglobulin, IgG2a

COA COA
    Application:
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2a
  • Clone Number: CD01/2B7
  • Conjugation: Unconjugated
In stock
Item Number
Ab169199
Grouped product items
SKU Size
Availability
 Price Qty
Ab169199-10μl
10μl
≥10
$99.90
Ab169199-50μl
50μl
10
$189.90
Ab169199-100μl
100μl
≥10
$299.90
Ab169199-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (CD01/2B7); Mouse anti Human beta 2 Microglobulin Antibody; WB, ELISA, ICC/IF; Unconjugated

View related series
Click to view target information: B2M
Accession#:P61769 (6) B2M (6) Beta 2 Microglobulin (4) Gene ID:567 (6)

Basic Description

Product Name beta 2 Microglobulin Mouse mAb - Primary antibody, specific to beta 2 Microglobulin, IgG2a
Synonyms B2M | B2MG_HUMAN | Beta 2 microglobin | Beta 2 microglobulin | Beta 2 microglobulin precursor | Beta chain of mhc class 1 proteins | Beta chain of MHC class I molecules | Beta-2-microglobulin form pI 5.3 | CDABP0092 | Hdcma22p | IMD43 | beta-2-microglobul
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL
Host species Mouse
Specificity beta 2 Microglobulin
Immunogen Recombinant protein corresponding to Human beta-2-microglobulin
Positive Control WB: HeLa, Raji, THP-1 and HepG2 Whole cell Lysates ICC/IF: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1/1,000 - 1/10,000; Predicted molecular weight: 13.7 KDa
ICC/IF: 1/200

Product Properties

Isotype IgG2a
Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

B2M Tbio Beta-2-microglobulin (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

beta 2 Microglobulin Mouse mAb (Ab169199) - ICC/IF
IF analysis of beta 2 Microglobulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta 2 Microglobulin Mouse mAb (Ab169199) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/200

WB

1/1,000 - 1/10,000; Predicted molecular weight: 13.7 KDa

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number Certificate Type Date Item
ZJ24F0404432 Certificate of Analysis Apr 11, 2024 Ab169199
ZJ24F0404431 Certificate of Analysis Apr 11, 2024 Ab169199
ZJ24F0404430 Certificate of Analysis Apr 11, 2024 Ab169199
ZJ24R0300410 Certificate of Analysis Mar 29, 2024 Ab169199

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