货号 (SKU)

包装规格

是否现货

价格

数量

S670007-500ml

500ml

现货

基本描述

英文名称

SDS-PAGE Separating Gel Buffer(4×)

储存温度

2-8°C储存

运输条件

冰袋运输

产品介绍

产品简介

本产品为配制SDS-PAGE分离胶缓冲液，可用于配制各种浓度的变性及非变性PAGE凝胶，方便、快捷。产品中已加入10%SDS,使用时不用另外加入。

操作步骤

根据目的蛋白分子量大小选择合适的PAGE分离胶配制浓度，最佳胶浓度请参考附表1。

I 灌制分离胶（各试剂使用量请参考附表2）

1．参照凝胶模具说明书，装配好凝胶模具。

2. 将不同体积的30%Acr-Bis(29:1)、SDS-PAGE Separating Gel Buffer（4×）分离胶缓冲液和纯水在小烧杯或试管中混合。

3. 加入10%APS和TEMED，轻轻搅拌使其混匀，避免产生气泡。

4. 在凝胶模具中灌入适量分离胶溶液（对于mini-gel，凝胶液加至约距前玻璃板顶端1.5 cm或距梳齿约0.5 cm即可），然后在分离胶溶液上轻轻覆盖一层1 cm的水层，使凝胶表面保持平整。

5. 静置30-60分钟，待分离胶和水层之间出现一个清晰的界面后，表面凝胶已聚合。

II 灌制浓缩胶（各试剂使用量请参考附表3）

1. 去除覆盖在分离胶上的水层。

2. 将不同体积的30%Acr-Bis(29:1)、浓缩胶缓冲液和纯水在一个小烧杯或试管中混合。

3. 加入10%过硫酸铵和TEMED，轻轻搅拌使其混匀，避免产生气泡。

4. 将浓缩胶溶液加至分离胶的上面，直至凝胶溶液到达前玻璃板的顶端。

5. 将梳子插入凝胶内，避免产生气泡。

6. 静置10~20分钟，等待浓缩胶聚合。

7. 待凝胶聚合后，小心地拔出梳子，以免破坏加样孔。

8. 进行常规电泳操作。

附表

附表1. SDS-PAGE分离胶的浓度与最佳分离范围

附表2. 配制SDS-PAGE分离胶

附表3. 配制5%SDS-PAGE浓缩胶

Products

This product is a buffer for the preparation of SDS-PAGE separation gels, which can be used to prepare denaturing and non-denaturing PAGE gels of various concentrations, convenient and quick. 10% SDS has been added to the product, so there is no need to add it separately.

Procedure

According to the molecular weight size of the target protein, select the appropriate concentration of PAGE separation gel preparation, the optimal gel concentration, please refer to Exhibit 1.

I Infusion of separating gel (please refer to Schedule 2 for the amount of each reagent used)

1. Refer to the gel mold instructions and assemble the gel mold.

2. Mix different volumes of 30% Acr-Bis (29:1), SDS-PAGE Separating Gel Buffer (4×) Separating Gel Buffer and pure water in a small beaker or test tube.

3. Add 10% APS and TEMED, stir gently to mix well and avoid air bubbles.

4. In the gel mold filled with the appropriate amount of separation gel solution (for mini-gel, the gel solution added to about 1.5cm from the top of the front glass plate or about 0.5cm from the teeth of the comb can be), and then gently covered with a layer of 1cm of water on the separation gel solution, so that the surface of the gel to maintain a flat.

5. Let it stand for 30-60 minutes, after a clear interface appears between the separated gel and the water layer, the surface gel has been polymerized.

II Filling concentrated gel (please refer to Exhibit 3 for the amount of each reagent used)

1. Remove the water layer covering the separator gel.

2. Mix different volumes of 30% Acr-Bis (29:1), concentrated gel buffer and pure water in a small beaker or test tube.

3. Add 10% ammonium persulfate and TEMED, stir gently to mix well and avoid air bubbles.

4. Add the concentrated gel solution to the top of the separation gel until the gel solution reaches the top of the front glass plate.

5. Insert the comb into the gel to avoid air bubbles.

6. Let it stand for 10~20 minutes and wait for the concentrated gel to polymerize.

7. After the gel has polymerized, carefully pull out the comb so as not to damage the spiking hole.

8. Perform routine electrophoresis operations.

Schedules

Exhibit 1. Concentration of SDS-PAGE Separation Gel and Optimal Separation Range

SDS-PAGE separation gel concentration	Optimal separation range
6%gel	50-150 kD
8%gel	30-90 kD
10%gel	20-80 kD
12%gel	12-60 kD
15%gel	10-40 kD

Schedule 2. Preparation of SDS-PAGE Separation Gel

Separation gel concentration	Gel volume	Required volume of each component (unit: ml)
		pure water	30%Acr-Bis(29:1)	Separation gel buffer (4x)	10%APS	TEMED
6%	5 ml	2.75	1.0	1.25	0.05	0.004
	10 ml	5.5	2.0	2.5	0.1	0.008
	15 ml	8.25	3.0	3.75	0.15	0.012
	20 ml	11	4.0	5	0.2	0.016
8%	5 ml	2.42	1.33	1.25	0.05	0.003
	10 ml	4.8	2.7	2.5	0.1	0.006
	15 ml	7.25	4.0	3.75	0.15	0.009
	20 ml	9.7	5.3	5	0.2	0.012
10%	5 ml	2.08	1.67	1.25	0.05	0.002
	10 ml	4.17	3.33	2.5	0.1	0.004
	15 ml	6.25	5.0	3.75	0.15	0.006
	20 ml	8.3	6.7	5	0.2	0.008
12%	5 ml	1.75	2.0	1.25	0.05	0.002
	10 ml	3.5	4.0	2.5	0.1	0.004
	15 ml	5.25	6.0	3.75	0.15	0.006
	20 ml	7.0	8.0	5	0.2	0.008
15%	5 ml	1.25	2.5	1.25	0.05	0.002
	10 ml	2.5	5.0	2.5	0.1	0.004
	15 ml	3.75	7.5	3.75	0.15	0.006
	20 ml	5	10.0	5	0.2	0.008

Schedule 3. Preparation of 5% SDS-PAGE gel concentrate

Gel volume	Required volume of each component (unit: ml)
	pure water	30%Acr-Bis(29:1)	Concentrated gel buffer (4x)	10%APS	TEMED
2 ml	1.14	0.34	0.5	0.02	0.002
4 ml	2.28	0.68	1	0.04	0.004
6 ml	3.42	1.02	1.5	0.06	0.006
8 ml	4.56	1.36	2.0	0.08	0.008

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批号(Lot Number)	证书类型	货号
E2408247	分析证书	S670007

技术文档和文章

s670007_sds-page separating gel buffer(4×).pdf

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溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

SDS-PAGE分离胶缓冲液（4×）

库存信息

基本描述

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器