Recombinant Human IL-6 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: P05231
蛋白标签: N-His
生物活性: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.123 ng/mL. Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 μg/mL can bind MEDI-5117 (anti-IL-6) (Ab182698) with the EC50 of 13.84 ng/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

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货号 (SKU)	包装规格	是否现货
rp156005-10μg	10μg	现货
rp156005-50μg	50μg	现货
rp156005-100μg	100μg	现货
rp156005-1mg	1mg	期货

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点击查看蛋白信息: IL6

Accession#:P05231 (23) Gene ID:3569 (23) IL-6 (9) IL6 (25)

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基本描述

产品名称	Recombinant Human IL-6 Protein
别名	重组人白细胞介素-6蛋白 \| 重组人IL-6蛋白 \| 重组人 IL-6 蛋白
英文别名	Interleukin BSF 2 \| B cell differentiation factor \| B cell stimulatory factor 2 \| B-cell stimulatory factor 2 \| BSF 2 \| BSF-2 \| BSF2 \| CDF \| CTL differentiation factor \| Cytotoxic T cell differentiation factor \| Hepatocyte stimulatory factor \| Hepatocyte
规格或纯度	ActiBioPure™, Bioactive, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining Description: Interleukin-6 (IL-6) is a pleiotropic, alpha-helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand-binding subunit (IL-6 R alpha) and a signal-transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. Gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.
生物活性	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.123 ng/mL. Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 μg/mL can bind MEDI-5117 (anti-IL-6) (Ab182698) with the EC50 of 13.84 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	30-212 aa
序列	MGSSHHHHHHSSGLVPRGSHMVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM
纯度	>95% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P05231
来源	重组表达
预测分子量	23.1 kDa
SDS-PAGE	24.1 kDa, under reducing condition

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IL-6 Protein (rp156005)

Recombinant Human IL-6 Protein (rp156005) - Protein Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.123 ng/mL.

SDS-PAGE: Recombinant Human IL-6 Protein (rp156005)

Recombinant Human IL-6 Protein (rp156005) - SDS-PAGE
3 μg/lane of Recombinant Human IL-6 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 24.1 kDa.

ELISA: Recombinant Human IL-6 Protein (rp156005)

Recombinant Human IL-6 Protein (rp156005) - ELISA
Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 μg/mL can bind MEDI-5117 (anti-IL-6) (Ab182698) with the EC ₅₀ of 13.84 ng/mL.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0606477	分析证书	rp156005
ZJ24F0606476	分析证书	rp156005
ZJ24F1216140	分析证书	rp156005
ZJ24F1216139	分析证书	rp156005

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Recombinant Human IL-6 Protein

库存信息

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基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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